PMID: 7543134Aug 1, 1995Paper

Cell cycle control of c-kit+IL-7R+ B precursor cells by two distinct signals derived from IL-7 receptor and c-kit in a fully defined medium

The Journal of Experimental Medicine
M YasunagaS Nishikawa

Abstract

An important goal for the investigation of the proliferation of mammalian cells is to establish a fully defined condition for culturing them in vitro. Here, we report establishment of a fully defined culture condition that supports the primary culture of normal c-kit+IL-7 receptor (IL-7R)+ B precursor cells without the aid of stromal cell lines. This defined culture condition contains IL-7, the ligand for c-kit, transferrin, insulin, and bovine serum albumin as protein components. By using the cell lines derived from RAG2(-/-) mice, which do not differentiate into c-kit- stage, we have evaluated the role of each protein in the cell cycle progression of c-kit+IL-7R+ B precursor cells. Since B precursor cells can grow without insulin, c-kit remains a sole functional receptor tyrosine kinase for their growth. While both c-kit ligand (KL) and IL-7 are the requisite molecules for sustained proliferation of B precursor cells, each molecule plays distinct roles. IL-7 starvation results in prompt arrest of the cells at G1. An accumulation of the cells in the mitotic phase was also detected. Thus, the major role of IL-7 is to regulate the G1/S transition and the process of cytokinesis of B precursor cells. Although prolonged KL starvati...Continue Reading

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Citations

Apr 1, 1996·Current Opinion in Immunology·M J Owen, A R Venkitaraman
May 14, 1999·Current Opinion in Immunology·A M BairdL J Berg
Jan 1, 1996·The Journal of Cell Biology·M YasunagaS Nishikawa
Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·M Dorsch, S P Goff
Apr 1, 1997·Leukemia & Lymphoma·K W SykoraA Reiter
Jan 1, 1996·Immunity·S Delassus, A Cumano
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Jun 11, 2019·Seminars in Cancer Biology·Masahiro Yasunaga

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