Cell cycle control of spindle pole body duplication and splitting by Sfi1 and Cdc31 in fission yeast

Journal of Cell Science
Imène B BouhlelAnne Paoletti

Abstract

Spindle pole biogenesis and segregation are tightly coordinated to produce a bipolar mitotic spindle. In yeasts, the spindle pole body (SPB) half-bridge composed of Sfi1 and Cdc31 duplicates to promote the biogenesis of a second SPB. Sfi1 accumulates at the half-bridge in two phases in Schizosaccharomyces pombe, from anaphase to early septation and throughout G2 phase. We found that the function of Sfi1-Cdc31 in SPB duplication is accomplished before septation ends and G2 accumulation starts. Thus, Sfi1 early accumulation at mitotic exit might correspond to half-bridge duplication. We further show that Cdc31 phosphorylation on serine 15 in a Cdk1 (encoded by cdc2) consensus site is required for the dissociation of a significant pool of Sfi1 from the bridge and timely segregation of SPBs at mitotic onset. This suggests that the Cdc31 N-terminus modulates the stability of Sfi1-Cdc31 arrays in fission yeast, and impacts on the timing of establishment of spindle bipolarity.

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Citations

Jan 19, 2016·Biochimica Et Biophysica Acta·Juan Martinez-Sanz, Liliane Assairi
Jun 17, 2015·The Journal of Cell Biology·Christian SeyboldElmar Schiebel
Jan 25, 2017·Science Advances·Robert BlackwellMeredith D Betterton
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Feb 2, 2021·The Journal of Cell Biology·Diana RüthnickElmar Schiebel
Apr 27, 2021·Frontiers in Cell and Developmental Biology·Masamitsu SatoMika Toya

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