Cell cycle dependent aneuploidy induction by X-rays in vitro in human lymphocytes

Microscopy Research and Technique
I TallonM Kirsch-Volders

Abstract

Although ionising radiation mainly induces DNA strand breaks leading to chromosomal aberrations, there are indications that it also might induce numerical chromosome aberrations (aneuploidy). The existing data, however, do not provide evidence for a mechanism. To assess the relative sensitivity of the G1 vs. G2 cellular targets, whole blood cultures of lymphocytes were irradiated in vitro with different doses of X-rays (0.5, 1 and 2 Gy). The lymphocytes were harvested after cytochalasin-B blockade to allow the selective study of binucleated cells, having undergone only one division in culture. Harvesting was performed at different sampling times (70, 74, and 78 hours). To evaluate the micronuclei, regarding whole chromosomes or acentric fragments, an oligonucleotide probe that recognises the centromeric region of all human chromosomes was used. The relative percentage of centromere-positive micronuclei ranged from 5 up to 18% depending on the cell cycle stage and on the received dose. Cells exposed during the G1 phase exhibited a slightly higher frequency of centromere-positive micronuclei than cells that were in G2 at the time of exposure. G1 exposure induced a centromere-positive micronuclei dose-effect relationship that was ...Continue Reading

References

Jan 1, 1991·Environmental and Molecular Mutagenesis·J A HeddleJ T MacGregor
Mar 1, 1990·Mutagenesis·P Van Hummelen, M Kirsch-Volders
Jan 1, 1986·Annual Review of Biophysics and Biophysical Chemistry·J W Gray, R G Langlois
Jan 1, 1985·Basic Life Sciences·J C Liang, B R Brinkley
May 1, 1973·Mutation Research·J A Heddle
Jul 15, 1983·Science·J J Yunis
Jan 1, 1981·Journal of Immunological Methods·G D Johnson, G M Nogueira Araujo
Jun 1, 1994·Mutation Research·J M LeeA Bernstein
Apr 1, 1994·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·A MaityR J Muschel

❮ Previous
Next ❯

Citations

Jul 6, 2004·International Journal of Radiation Oncology, Biology, Physics·Timothy M Pawlik, Khandan Keyomarsi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.