Cell Cycle-Dependent Localization of Dynactin Subunit p150 glued at Centrosome

Journal of Cellular Biochemistry
Ting-Yu ChenChia-Yih Wang

Abstract

p150(glued) is the largest subunit of dynactin protein complex, through which cargo vesicles link to the microtubule minus-end directed motor protein dynein. In addition, p150(glued) also locates in the mother centriole where it organizes the subdistal appendage. The components of appendage are dynamically regulated throughout the cell cycle stages, but it is still unclear whether the centrosomal residency of p150(glued) correlated with cell cycle progression. Here we found that p150(glued) was located in the mother centriole during G1/S stage and its centrosomal residency was independent of microtubule transportation. However, the centrosomal p150(glued) became blurred at G2/M phase and this event was not regulated by its phosphorylation. Entering into mitosis, p150(glued) was robustly enriched in the mitotic spindle nearby the spindle poles but not in the centrosome. During serum starvation (G0 stage), p150(glued) appeared at the base of primary cilium and its depletion attenuated starvation-induced primary cilium formation. We also checked its role in the maintenance of centrosome homeostasis and configuration, and found depletion of p150(glued) did not induce centrosome amplification or splitting but inhibited U2OS cell gro...Continue Reading

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Citations

Aug 2, 2018·Biochemical Society Transactions·Anthony J Roberts
Nov 4, 2016·Journal of Cell Science·Daniel J TarrantCampbell W Gourlay
Dec 28, 2017·Journal of Cellular Biochemistry·Wei-Chih LienChia-Yih Wang
Oct 24, 2019·International Journal of Molecular Sciences·Chia-Yih WangPei-Yin Tsai
Feb 15, 2021·Biochimica Et Biophysica Acta. Reviews on Cancer·J PriyangaDipita Bhakta-Guha

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