Cell-cycle deregulation induced by three different root canal sealers in vitro

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics
Caroline R A Valois, Ricardo B Azevedo

Abstract

The aim of this study was to investigate the effects of 3 root canal sealers on the cell cycle of mouse fibroblasts (3T3 cell line). Freshly mixed root canal sealers (AH Plus, Endofill, and Sealer 26) were eluted for 24 h using cell culture medium. Antiproliferation activity of different eluate dilutions was determined by 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide assay. Cell cycle was subsequently evaluated by flow cytometry. Differences were tested by analysis of variance with Tukey test (P < .05). Results showed that the root canal sealers inhibited cell growth in a dose-dependent manner. Antiproliferation activity of the root canal sealers was associated with cell-cycle deregulation. This event involved cell-cycle arrest in the S/G2/M phases and an increase of the sub-G1 population, which are consistent with both cytostatic and cytotoxic effects. This information may contribute to a better understanding of the toxicity mechanism displayed by root canal sealers currently used in clinical practice.

References

Apr 20, 2001·Seminars in Hematology·Z DarzynkiewiczP Smolewski
Sep 25, 2004·Journal of Materials Science. Materials in Medicine·Tsui-Hsien HuangChia-Tze Kao
Aug 22, 2007·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Saziye Sari, Leyla Duruturk
Nov 27, 2007·Journal of Endodontics·Melahat GorduysusAli Ugur Ural

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Citations

Dec 11, 2019·Materials·Diogo Afonso FonsecaManuel Marques Ferreira

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