PMID: 7540181Mar 1, 1995Paper

Cell cycle genes c-mos and cyclin-B1 are expressed in a specific pattern in human oocytes and preimplantation embryos

Human Reproduction
O HeikinheimoW E Gibbons

Abstract

Little is known about the molecular mechanisms governing the development of human oocyte and pre-embryo. We characterized the expression pattern of c-mos, cyclin B1 and beta-actin mRNA in oocytes and granulosa cells from human and monkey, and in human early embryos, using both qualitative and semi-quantitative reverse transcriptase polymerase chain reaction. The proto-oncogene c-mos was expressed in an oocyte-specific manner and no mRNA for c-mos could be detected in the granulosa cells. Similarly, strong expression of cyclin-B1 was seen in the oocytes. In human pre-embryos, the expression of cyclin-B1 and beta-actin increased from the 6-cell stage onwards, indicating active transcription and thus activation of embryonic genome either at or before the 6-cell stage. The expression of c-mos was transient and very little c-mos mRNA could be detected in the human embryos beyond the 6-cell stage. Thus, both its time-specific and site-specific expression suggest meiosis-specific functions for the proto-oncogene c-mos in human oocytes. As judged by the disappearance of c-mos, the maternal pool of mRNA seems to be degraded towards the 6- to 8-cell stage. The transient expression of c-mos and high levels of cyclin-B1 mRNA suggest that m...Continue Reading

Citations

Jan 1, 1999·Maturitas·U Eichenlaub-Ritter
Mar 3, 2004·Reproductive Biomedicine Online·Ri-Cheng ChianSeang-Lin Tan
Mar 31, 2006·Human Reproduction·Said AssouSamir Hamamah
Aug 1, 1997·The Journal of Obstetrics and Gynaecology Research·K HanaiY Tomoda
Apr 12, 2011·The Journal of Reproduction and Development·Rebecca R PaytonJ Lannett Edwards
Oct 2, 2009·Journal of Endocrinological Investigation·B Meczekalski
Jan 12, 1999·Developmental Biology·G D SmithD P Wolf
Aug 28, 2015·Molecular Biology of the Cell·Neil R AdamesJean Peccoud
Nov 7, 2002·Molecular Reproduction and Development·Xia WangGary D Smith
Oct 29, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·Xia WangGary D Smith
Jan 6, 2007·Human Cell·Toshio HamataniYasunori Yoshimura
Feb 9, 1999·Prenatal Diagnosis·E Pergament, M Fiddler
Dec 4, 2003·Molecular Reproduction and Development·Alexandra Zilz, Geoffrey M Cooper
Aug 4, 2018·Journal of Cellular Biochemistry·Feifei ShiCong Zhang

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