Cell-cycle, phase-specific activation of Maize streak virus promoters

Molecular Plant-microbe Interactions : MPMI
K NikovicsG V Horváth

Abstract

It is believed that geminiviral DNA replication is coupled to the cell-cycle regulatory complex of the plant cell and that the virus-early (complementary or C sense) gene products REP and REPA may be able to manipulate the regulation of the cycle. In this study, we examined expression from the promoters of Maize streak virus (MSV) in transgenic maize plants and cells to determine whether they showed cell-cycle specificity. Histochemical staining of plant roots containing "long and short" C-sense promoter sequences upstream of the GUS (beta-glucuronidase) reporter gene showed that promoter activity was restricted to the meristematic region of the roots and was enhanced by 2,4-dichlorophenoxy acetic acid (2,4-D) treatment. Analysis of reporter gene and cell-cycle-specific gene transcript levels coupled with flow cytometric data in synchronized transgenic maize cells revealed that all of the MSV promoters showed cell-cycle specificity. The coat protein gene promoter showed highest activity in early G2, whereas the C-sense promoter sequences produced two peaks of activity in the S and G2 cell-cycle phases.

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Citations

Jul 31, 2002·Annual Review of Phytopathology·Godelieve Gheysen, Carmen Fenoll
Feb 26, 2005·Virology Journal·Jeff VeltenChristopher I Cazzonelli
Oct 26, 2005·PLoS Genetics·Darren P MartinEdward P Rybicki
May 8, 2010·Molecular Plant Pathology·Carolina EscobarCarmen Fenoll
May 18, 2016·Virology·B K BorahI Dasgupta

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