PMID: 9811697Nov 13, 1998

Cell cycle progression in monkey cells expressing simian virus 40 small t antigen from adenovirus vectors

Journal of Virology
A HoweK Rundell

Abstract

The simian virus 40 small t antigen (small-t) is required for optimal viral replication and transformation, especially during the infection of nondividing cells, suggesting that the function of small-t is to promote cell cycle progression. The mechanism through which small-t promotes cell growth reflects, in part, its binding and inhibition of protein phosphatase 2A (PP2A). The use of recombinant adenoviruses allows small-t expression in a majority of cells in a population, thus providing a convenient source of cells for biochemical analyses. In monkey kidney CV1 cells, small-t expressed from these adenovirus vectors activated the mitogen-activated protein kinase (MAPK) pathway, induced JNK activity, and increased AP-1 DNA-binding activity, all in a PP2A-dependent manner. Expression of small-t also caused an increase in the phosphorylation of the Na+/H+ antiporter, a mitogen-activated ion exchanger whose activity correlates with its phosphorylation. At least part of the antiporter phosphorylation induced by small-t reflected activation of the MAPK pathway, as suggested by results of assays using a chemical inhibitor of the MAPK-activating kinase, MEK. Finally, small-t expression from adenovirus vectors promoted efficient cell c...Continue Reading

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Citations

May 1, 2010·Future Virology·A Sami SaribasMahmut Safak
Mar 17, 2017·Clinical Microbiology Reviews·George R AmbalathingalRajiv Khanna
Sep 5, 2001·Journal of Virology·O GjoerupT M Roberts
Nov 21, 2007·Journal of Cellular Physiology·Kamel KhaliliMahmut Safak
Feb 14, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Qinghang Liu, Polly A Hofmann
Jan 25, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Juan Carlos CaraballoAlejandro P Comellas
Jan 25, 2008·Cancer Metastasis Reviews·Anna A Sablina, William C Hahn

Related Concepts

MAP3K1 protein, human
Adenoviruses
Antigens, Neoplasm, Viral
Cell Cycle
Cell Division Phases
Cercopithecus tantalus
Enzyme Activation
Shuttle Vectors
Protein-Threonine Phosphatase
Phosphorylation

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