PMID: 9811697Nov 13, 1998

Cell cycle progression in monkey cells expressing simian virus 40 small t antigen from adenovirus vectors

Journal of Virology
A HoweK Rundell


The simian virus 40 small t antigen (small-t) is required for optimal viral replication and transformation, especially during the infection of nondividing cells, suggesting that the function of small-t is to promote cell cycle progression. The mechanism through which small-t promotes cell growth reflects, in part, its binding and inhibition of protein phosphatase 2A (PP2A). The use of recombinant adenoviruses allows small-t expression in a majority of cells in a population, thus providing a convenient source of cells for biochemical analyses. In monkey kidney CV1 cells, small-t expressed from these adenovirus vectors activated the mitogen-activated protein kinase (MAPK) pathway, induced JNK activity, and increased AP-1 DNA-binding activity, all in a PP2A-dependent manner. Expression of small-t also caused an increase in the phosphorylation of the Na+/H+ antiporter, a mitogen-activated ion exchanger whose activity correlates with its phosphorylation. At least part of the antiporter phosphorylation induced by small-t reflected activation of the MAPK pathway, as suggested by results of assays using a chemical inhibitor of the MAPK-activating kinase, MEK. Finally, small-t expression from adenovirus vectors promoted efficient cell c...Continue Reading


May 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·N BouckG di Mayorca
Dec 1, 1992·Current Opinion in Cell Biology·N G AhnE G Krebs
Apr 1, 1991·Molecular and Cellular Biology·S I YangM C Mumby
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·G WalterM C Mumby
Jan 1, 1989·Annual Review of Biochemistry·P Cohen
Aug 1, 1988·Molecular and Cellular Biology·Y W ChoiS R Ross
Aug 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J PouysségurS Paris
Mar 1, 1984·Virology·L S ChangG di Mayorca
Mar 1, 1984·The Journal of Cell Biology·C P Burns, E Rozengurt
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·D T DudleyA R Saltiel
Jan 1, 1995·The Journal of Physiology·C H YunM Donowitz
Jul 1, 1994·Trends in Biochemical Sciences·W L Kelley, S J Landry
Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·X Q QinP D Adams
Sep 1, 1994·Molecular and Cellular Biology·J A FrostJ R Feramisco
Jan 1, 1995·Methods in Enzymology·C A Lange-Carter, G L Johnson
May 24, 1996·Journal of Molecular Biology·S Swaminathan, B Thimmapaya
Apr 1, 1996·Kidney International·L Bianchini, J Pouysségus
Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·G WatanabeR G Pestell
Nov 1, 1996·The American Journal of Physiology·C BooksteinE B Chang
Oct 6, 1997·The EMBO Journal·M W RenshawM A Schwartz


May 1, 2010·Future Virology·A Sami SaribasMahmut Safak
Mar 17, 2017·Clinical Microbiology Reviews·George R AmbalathingalRajiv Khanna
Sep 5, 2001·Journal of Virology·O GjoerupT M Roberts
Nov 21, 2007·Journal of Cellular Physiology·Kamel KhaliliMahmut Safak
Feb 14, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Qinghang Liu, Polly A Hofmann
Jan 25, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Juan Carlos CaraballoAlejandro P Comellas
Jan 25, 2008·Cancer Metastasis Reviews·Anna A Sablina, William C Hahn

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