PMID: 9193070May 1, 1997Paper

Cell-cycle-regulated transcription of A- and B-type plant cyclin genes in synchronous cultures

The Plant Journal : for Cell and Molecular Biology
M ItoA Watanabe

Abstract

Synchronously dividing cell cultures of Catharanthus roseus were used to isolate cDNAs for two mitotic cyclins, named CYS and CYM. The deduced protein sequence of CYS is similar to that of A-type cyclins, and CYM belongs to the group of B-type cyclins. In a fashion similar to the pattern of expression seen for A-type and B-type cyclins in mammalian cells, CYS is expressed before CYM in C. roseus cells during the cell cycle. CYS mRNA accumulated at the onset of S phase and disappeared early in the G2 phase, whereas CYM mRNA was detected in the G2 and M phases of the cell cycle. Tobacco homologs of the two genes showed similar cell-cycle dependent expression patterns in synchronous cultures of tobacco BY2 cells. In both systems, CYS was expressed much earlier in the cell cycle than most other plant A-type cyclins, and hence CYS along with the soybean cyc1GM can be classified into a distinct subclass. The activities of CYM and CYS promoters during the cell cycle were analyzed in stably transformed tobacco BY2 cells. Cyclin promoter sequences of 0.5 kb could confer the typical cell-cycle-dependent expression to the beta-glucuronidase (GUS) reporter gene: the CYS promoter directed S-phase-specific expression, whereas the CYM promote...Continue Reading

Citations

Jan 13, 1998·Current Opinion in Cell Biology·J Doonan, P Fobert
May 10, 2002·The Plant Journal : for Cell and Molecular Biology·Margit Menges, James A H Murray
Sep 25, 2003·Annual Review of Plant Biology·Walter Dewitte, James A H Murray
Mar 3, 1999·Bioscience, Biotechnology, and Biochemistry·N Q PalacpacT Seki
Sep 14, 2004·The Plant Journal : for Cell and Molecular Biology·Yasuhiro KadotaKazuyuki Kuchitsu
Nov 4, 2006·Plant, Cell & Environment·Kaoru SuzukiHideaki Shinshi
Jul 6, 2000·FEBS Letters·S PlanchaisC Bergounioux
Nov 26, 1999·FEBS Letters·M SekineA Shinmyo
Aug 10, 2002·The Journal of Biological Chemistry·Margit MengesJames A H Murray
Apr 1, 2003·The Journal of Biological Chemistry·Ziyin Li, Ching C Wang
Jan 10, 2021·Biochimie·Aurora Lara-NúñezJorge M Vázquez-Ramos
Dec 17, 2020·International Journal of Molecular Sciences·Juan MengHengbin He

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