Cell cycle regulation of pulmonary phosphatidylcholine synthesis

American Journal of Respiratory Cell and Molecular Biology
Irene TseuMartin Post

Abstract

Pulmonary surfactant phosphatidylcholine (PC) formation increases as alveolar type II cells mature and arrest in G0/G1 state of the cell cycle at late fetal gestation. To determine whether this G0/G1 arrest is responsible for the increase in PC synthesis, we investigated the rates of PC synthesis and the activity, phosphorylation, intracellular distribution, synthesis, and degradation of a key enzyme of PC synthesis, cytidine triphosphate (CTP):phosphocholine cytidylyltransferase (CCTalpha). In synchronized mouse lung epithelial (MLE)-15 cells, PC production and CCTalpha activity peaked at G0/G1, declined during transition to G1/S, and remained low during S and G2/M. The changes in CCTalpha activity were not due to alterations in CCTalpha gene and protein expression. CCTalpha protein degradation also did not change during the cell cycle. Indirect immunofluorescence and immunogold electron microscopy revealed that CCTalpha localized to the cytoplasmic compartment and that its cytosolic localization did not change with the cell cycle. Although immunoblotting suggested no major redistribution of CCTalpha mass from cytosol to endoplasmic reticulum, activity measurements revealed that the ratio of particulate/soluble CCTalpha activi...Continue Reading

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Citations

Nov 30, 2006·Molecular and Cellular Biology·Yong TianSuzanne Jackowski
Feb 8, 2003·American Journal of Respiratory and Critical Care Medicine·Antonia FehrenbachHeinz Fehrenbach
Aug 18, 2009·American Journal of Respiratory Cell and Molecular Biology·Ross RidsdaleMartin Post
Mar 9, 2011·Progress in Lipid Research·Martin HermanssonPentti Somerharju
Jul 22, 2010·Biochimica Et Biophysica Acta·Nicolás O FavaleNorma B Sterin-Speziale
Feb 26, 2008·Progress in Lipid Research·Hiroyuki SugimotoDennis E Vance
Sep 27, 2012·Biochimica Et Biophysica Acta·Paolo Fagone, Suzanne Jackowski
Dec 4, 2012·Biochimica Et Biophysica Acta·Victoria GossAnthony D Postle
Nov 23, 2006·The International Journal of Biochemistry & Cell Biology·Weiyang Lin, Gilbert Arthur
Jun 1, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ross Ridsdale, Martin Post
Mar 31, 2004·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Jean E Vance, Dennis E Vance
Nov 13, 2004·The Journal of Biological Chemistry·Suzanne Jackowski, Paolo Fagone
Jul 19, 2006·Canadian Journal of Physiology and Pharmacology·Alicia G Howe, Christopher R McMaster
Sep 24, 2004·The Journal of Biological Chemistry·Alex D Manguikian, Suzanne E Barbour
Mar 25, 2005·The Journal of Biological Chemistry·Marianna AgassandianRama K Mallampalli

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