Cell cycle specificity of certain antimicrotubular drugs in Schizosaccharomyces pombe

Journal of General Microbiology
G M Walker

Abstract

Of the seven antimicrotubular drugs tested, nocodazole, mebendazole and trifluralin at saturable concentrations failed to inhibit cell division in Schizosaccharomyces pombe, while carbendazim, thiabendazole and chloropropham each at 50 micrograms ml- and amiprophos methyl at 200 micrograms ml-1 completely arrested cell division. This inhibition was associated with striking morphological changes in which carbendazim- and thiabendazole-treated cells became elongated and pseudohyphal, whereas chloropropham- and amiprophos methyl-treated cells appeared small and rounded with occasional V-shaped pairs. Lomofungin staining revealed that nuclear division was also arrested by these drugs. Suspected blockage of defined cell cycle stages was confirmed by pulse-induction experiments which revealed that cells could be synchronized into division using exposure to a drug for one generation. Further experiments with synchronous cultures prepared by size selection showed that different drugs possessed different transition points; for example, carbendazim and thiabendazole were effective in blocking a late stage of the cell cycle just prior to division, whereas amiprophos methyl affected a very early stage. The results suggest that some of the ...Continue Reading

Citations

Feb 27, 1999·Current Opinion in Cell Biology·G G Gundersen, T A Cook
Mar 6, 1999·Current Opinion in Microbiology·F Verde
Feb 14, 2012·Nature Chemical Biology·Sarah A WackerTarun M Kapoor
May 22, 2001·Molecular Biology of the Cell·H Jin, D C Amberg
Dec 29, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·D Brunner, P Nurse
Jun 15, 2010·PloS One·Mingyu LiuWilliam P Halford
Jul 22, 1997·Proceedings of the National Academy of Sciences of the United States of America·X HeS Sazer
Aug 22, 2001·Molecular Biology of the Cell·T W HendricksonH C Joshi
Jul 29, 1998·The Journal of Cell Biology·K E Sawin, P Nurse
Nov 22, 2000·The Journal of Cell Biology·U FleigS Sazer
Apr 20, 2001·The Journal of Cell Biology·P T TranF Chang
Jan 22, 2015·Proceedings of the National Academy of Sciences of the United States of America·Alexandra SurcelDouglas N Robinson
Feb 4, 1999·European Journal of Cell Biology·G Steinberg, J R McIntosh
Jan 5, 2011·Taiwanese Journal of Obstetrics & Gynecology·Chi-Chen ChangChih-Sheng Lin
Dec 13, 1997·Canadian Journal of Microbiology·A Svoboda, I Slaninová
Jun 27, 2007·Journal of Cell Science·Stefania CastagnettiP Nurse

Related Concepts

FB642
Amiprophos methyl
Cochliobolus
Benzimidazoles
Carbamic Acids
Cell Cycle
Cell Division Phases
Chlorpropham
Vermin
Microtubules

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