Cell cycle synchronization and BrdU incorporation as a tool to study the possible selective elimination of ErbB1 gene in the micronuclei in A549 cells

Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas
Camila LauandGláucia Maria Machado-Santelli

Abstract

Lung cancer often exhibits molecular changes, such as the overexpression of the ErbB1 gene that encodes epidermal growth factor receptor (EGFR). ErbB1 amplification and mutation are associated with tumor aggressiveness and low response to therapy. The aim of the present study was to design a schedule to synchronize the cell cycle of A549 cell line (a non-small cell lung cancer) and to analyze the possible association between the micronuclei (MNs) and the extrusion of ErbB1 gene extra-copies. After double blocking, by the process of fetal bovine serum deprivation and vincristine treatment, MNs formation was monitored with 5-bromo-2-deoxyuridine (BrdU) incorporation, which is an S-phase marker. Statistical analyses allowed us to infer that MNs may arise both in mitosis as well as in interphase. The MNs were able to replicate their DNA and this process seemed to be non-synchronous with the main cell nuclei. The presence of ErbB1 gene in the MNs was evaluated by fluorescent in situ hybridization (FISH). ErbB1 sequences were detected in the MNs, but a relation between the MNs formation and extrusion of amplified ErbB1 could not be established. The present study sought to elucidate the meaning of MNs formation and its association wit...Continue Reading

References

Sep 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·D D Von HoffG M Wahl
Sep 1, 1994·Mutation Research·G M Machado-SantelliC A Pereira
Dec 1, 1993·The Japanese Journal of Human Genetics·Y WangN Shimizu
Mar 28, 1998·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·I M AmbrosP F Ambros
Jul 17, 1998·Experimental Cell Research·R UzbekovY Arlot-Bonnemains
Jul 14, 2001·Histochemistry and Cell Biology·R Manelli-Oliveira, G M Machado-Santelli
Mar 22, 2003·Experimental Cell Research·Robert N JorissenAntony W Burgess
Feb 26, 2004·Bioscience, Biotechnology, and Biochemistry·Akiko SaitoHiroyuki Sunagawa
Nov 16, 2006·Genes, Chromosomes & Cancer·R NarathP F Ambros
Nov 23, 2006·Seminars in Cancer Biology·Fabien Kuttler, Sabine Mai
Feb 19, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·David A EberhardUNKNOWN Non-Small-Cell Lung Cancer Working Group
Mar 10, 2009·Cancer Treatment Reviews·Jaume CapdevilaJosep Tabernero
Dec 18, 2010·Mutagenesis·Yun HuangQinghua Shi
May 25, 2013·Journal of Experimental & Clinical Cancer Research : CR·Evandro Luís de Oliveira Niero, Gláucia Maria Machado-Santelli
Apr 30, 2014·Journal of Experimental & Clinical Cancer Research : CR·Evandro Luís NieroGláucia Maria Machado-Santelli

❮ Previous
Next ❯

Citations

Jun 15, 2016·Biochemistry. Biokhimii︠a︡·O P Kisurina-EvgenievaG E Onishchenko

❮ Previous
Next ❯