Cell death in the avian sclerotome

Developmental Biology
Esmond J Sanders

Abstract

In this study the occurrence of apoptotic cells in chick embryo trunk somites, between 2.5 and 4 days of development, has been examined using an in situ nick-end-labeling method (TUNEL) to identify nuclei in which DNA is undergoing fragmentation. At 2.5 days of development, apoptotic cells were found in the sclerotome with a distribution that depended on the rostrocaudal level in the trunk. At the most rostral levels (somites 1-18), dying cells were present primarily in the rostral half of the ventral sclerotome; at midlevels (somites 19-26), they were present throughout the ventral sclerotome; and at caudal levels (somites 27-32), no dying cells were present. By 4 days of development, the number of dying cells in the sclerotome was sharply reduced, and those present were primarily distributed to the caudal side of the intrasclerotomal fissure. Double labeling of cells for both TUNEL and the HNK-1 epitope, at 2.5 days, indicated that the majority of the dying cells were not neural crest cells. Further, dying cells in the rostral somite half were present largely in regions of the sclerotome that labeled poorly for HNK-1. It was confirmed that apoptotic neural crest cells retain the HNK-1 epitope and therefore would have been obs...Continue Reading

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Citations

May 26, 2005·Experimental Eye Research·Esmond J SandersSteve Harvey
Feb 26, 2014·Acta histochemica·Agnieszka K GrzegorzewskaHelena E Paczoska-Eliasiewicz
Aug 19, 2014·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Javier Francisco-MorcilloGervasio Martín-Partido
Jul 12, 2003·Birth Defects Research. Part A, Clinical and Molecular Teratology·Peter G Alexander, Rocky S Tuan
Jan 30, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·V Zuzarte-LuisJ M Hurle
Apr 15, 2020·Scientific Reports·Dana J RashidSusan C Chapman

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