Cell death induced by 131 I in a differentiated thyroid carcinoma cell line in vitro: necrosis or apoptosis?

Nuclear Medicine Communications
Kathrin MarxHarald Schicha

Abstract

The apoptotic and necrotic dose-response of thyroid carcinoma cells following irradiation with I was evaluated. In our in-vitro model, cells of well-differentiated papillary thyroid carcinoma (B-CPAP) were incubated with increasing activity concentrations of I for 2 days. Changes in cell viability and the extents of necrosis and apoptosis were evaluated both immediately and 2 days after irradiation. Viability of B-CPAP cells diminished with increasing I activity concentration. No apoptosis was detectable immediately after irradiation. Two days after irradiation significant apoptosis was found. The lowest I activity concentration at which apoptosis was detectable corresponds to about 1 MBq . ml. At higher activity concentrations a larger percentage of cells became apoptotic but the proportion decreased again at activity concentrations >10 MBq . ml. Likewise, necrosis was minimal at low activity concentrations and showed an exponential increase with rising I activity concentrations (>5-10 MBq . ml). Necrosis was already detectable immediately after irradiation and was the predominant form of cell death at high activity concentrations. The data suggest that the nature of the cytotoxic effect of I and whether it leads to apoptotic ...Continue Reading

References

Jul 1, 1991·The Annals of Physiological Anthropology = Seiri Jinruigaku Kenkyūkai Kaishi·J H AbrainiJ C Rostain
Apr 6, 1988·Journal of Immunological Methods·R Van Furth, T L Van Zwet
Mar 10, 1995·Science·H Steller
Apr 15, 1994·Cancer·J F KerrB V Harmon
Dec 24, 1997·Radiation and Environmental Biophysics·R MenzN E Crompton
Dec 6, 2000·European Journal of Nuclear Medicine·O SabriU Buell
Jul 7, 2001·Onkologie·B Pöllinger, E Dühmke
Jul 22, 2004·Nuclear Medicine Communications·Masud S HaqClive L Harmer
Dec 4, 2004·Nuclear Medicine Communications·Murat Faik ErdoğanNuri Kamel
Jul 8, 2005·Nuclear Medicine Communications·Mario Medvedec

❮ Previous
Next ❯

Citations

Mar 19, 2013·Revista española de medicina nuclear e imagen molecular·G KocaF Ornek
Apr 14, 2007·Cell Death and Differentiation·L GalluzziG Kroemer
Sep 6, 2019·International Journal of Radiation Biology·Andrew S RileyJohn Greenman
Jul 31, 2020·Current Eye Research·Gökhan KocaMeliha Korkmaz

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis