Abstract
Non-thermal plasmas are gaseous mixtures of molecules, radicals, and excited species with a small proportion of ions and energetic electrons. Non-thermal plasmas can be generated with any high electro-magnetic field. We studied here the pathological effects, and in particular cell death, induced by nanosecond-pulsed high voltage generated plasmas homogeneously applied on cell cultures and nude mouse skin. In vitro, Jurkat cells and HMEC exhibited apoptosis and necrosis, in dose-dependent manner. In vivo, on nude mouse skin, cell death occurred for doses above 113 J/cm(2) for the epidermis, 281 J/cm(2) for the dermis, and 394 J/cm(2) for the hypodermis. Using electron microscopy, we characterized apoptosis for low doses and necrosis for high doses. We demonstrated that these effects were not related to thermal, photonic or pH variations, and were due to the production of free radicals. The ability of cold plasmas to generate apoptosis on cells in suspension and, without any sensitizer, on precise skin areas, opens new fields of application in dermatology for extracorporeal blood cell treatment and the eradication of superficial skin lesions.
References
May 15, 1977·International Journal of Cancer. Journal International Du Cancer·U SchneiderG Bornkamm
Dec 1, 1992·The Journal of Investigative Dermatology·E W AdesT J Lawley
Jan 1, 1995·Archives of Dermatological Research·A MartinR R Anderson
Jun 26, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Stephen J BeebeKarl H Schoenbach
Oct 3, 2003·Biochemical and Biophysical Research Communications·P Thomas VernierMartin A Gundersen
Dec 4, 2003·Mutation Research·M StaceyS Buescher
Mar 4, 2008·Methods : a Companion to Methods in Enzymology·Dmitri V KryskoPeter Vandenabeele
Jan 30, 2009·The British Journal of Dermatology·T SmitsM-J P Gerritsen
Aug 12, 2009·Journal of the American Academy of Dermatology·Robert KnoblerHildegard Greinix
Jan 16, 2010·Interactive Cardiovascular and Thoracic Surgery·Martin HoffmannStefan Limmer
May 26, 2010·Journal of the European Academy of Dermatology and Venereology : JEADV·J HeinlinS Karrer
Aug 20, 2010·Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·Julia HeinlinSigrid Karrer
Nov 4, 2010·Annals of Biomedical Engineering·Rachel SensenigAri D Brooks
Oct 8, 2011·British Journal of Cancer·M KeidarB Trink
Nov 15, 2011·Bioelectrochemistry·Aude SilveLluis M Mir
Dec 24, 2011·Interactive Cardiovascular and Thoracic Surgery·Martin HoffmannPeter Kujath
Mar 28, 2012·International Review of Cell and Molecular Biology·Je-Ok Yoo, Kwon-Soo Ha
Apr 6, 2012·Bioelectrochemistry·James C WeaverT R Gowrishankar
Apr 7, 2012·The Journal of Surgical Research·Andrew S WuAri D Brooks
May 17, 2012·Expert Opinion on Biological Therapy·Massimo MartinoGiuseppe Bresolin
Sep 7, 2012·PloS One·Mahmoud Y AlkawareekBrendan F Gilmore
Jan 10, 2013·PloS One·Laura BrulléAlain Le Pape
May 25, 2013·PloS One·Julia KöritzerJürgen Schlegel
Jun 1, 2012·Medical Physics·J OnxleyO Craciunescu
Citations
Apr 25, 2014·PloS One·Yonghao MaKiwon Song
Dec 10, 2014·Biomolecules & Therapeutics·Beate HaertelUlrike Lindequist
May 2, 2015·Computers in Biology and Medicine·Robert Koprowski
Apr 6, 2017·PloS One·Spela KosUros Cvelbar
Aug 12, 2016·Journal of Tissue Engineering and Regenerative Medicine·Peter EisenhauerTheresa A Freeman
Jun 19, 2019·Scientific Reports·Marion BourdensValérie Planat-Bénard
Sep 28, 2017·Oxidative Medicine and Cellular Longevity·Eleonora TurriniCarmela Fimognari