Cell Death Is Not Sufficient for the Restriction of Potato Virus Y Spread in Hypersensitive Response-Conferred Resistance in Potato

Frontiers in Plant Science
Tjaša LukanKristina Gruden

Abstract

Hypersensitive response (HR)-conferred resistance to viral infection restricts the virus spread and is accompanied by the induction of cell death, manifested as the formation of necrotic lesions. While it is known that salicylic acid is the key component in the orchestration of the events restricting viral spread in HR, the exact function of the cell death in resistance is still unknown. We show that potato virus Y (PVY) can be detected outside the cell death zone inNy-1-mediated HR in potato plants (cv. Rywal), observed as individual infected cells or small clusters of infected cells outside the cell death zone. By exploiting the features of temperature dependentNy-1-mediated resistance, we confirmed that the cells at the border of the cell death zone are alive and harbor viable PVY that is able to reinitiate infection. To get additional insights into this phenomenon we further studied the dynamics of both cell death zone expansion and occurrence of viral infected cell islands outside it. We compared the response of Rywal plants to their transgenic counterparts, impaired in SA accumulation (NahG-Rywal), where the lesions occur but the spread of the virus is not restricted. We show that the virus is detected outside the cell de...Continue Reading

References

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Citations

Apr 6, 2019·Critical Reviews in Biotechnology·Ashish PrasadManoj Prasad
Sep 19, 2019·Phytopathology·Rawnaq N ChowdhuryAurélie M Rakotondrafara
Jul 10, 2019·Molecular Plant Pathology·Hernan Garcia-Ruiz
Nov 20, 2018·Frontiers in Plant Science·Dinesh Babu Paudel, Hélène Sanfaçon
Jan 18, 2020·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Lesley TorranceGlenn J Bryan
Mar 5, 2021·Bio-protocol·Katja StareKristina Gruden
Apr 24, 2021·Frontiers in Plant Science·Brian T RossMichelle L Flenniken
Jul 20, 2020··Andreja KežarMagda Tušek Žnidarič

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Methods Mentioned

BETA
transgenic
confocal microscopy
ELISA
temperature shift assay

Software Mentioned

Leica LAS X
R
R3

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