Cell death mechanisms in Leishmania amazonensis triggered by methylene blue-mediated antiparasitic photodynamic therapy

Photodiagnosis and Photodynamic Therapy
Débora P AurelianoMartha Simões Ribeiro

Abstract

Antiparasitic photodynamic therapy (ApPDT) is an emerging approach to manage cutaneous leishmaniasis (CL) since no side effects, contraindications and parasite resistance have been reported. In addition, methylene blue (MB) is a suitable photosensitizer to mediate ApPDT on CL. In this study we aimed to look for the best parameters to eradicate Leishmania amazonensis and investigated the cell death pathways involved in MB-mediated ApPDT. MB uptake by parasites was determined using different MB concentrations (50, 100, 250 and 500 μM) and incubation times (10, 30 and 60 min). L. amazonensis promastigotes were cultured and submitted to ApPDT using different concentrations of MB (50, 100 and 250 μM) combined to a red LED emitting at 645 ± 10 nm. The pre-irradiation time was 10 min. Two optical powers (100 mW and 250 mW) were tested and cells were exposed to 60 and 300 s of MB-mediated ApPDT delivering energies of 6, 15, 30 and 75 J and fluences of 21.2, 53.1, 106.2 and 265.4 J/cm2, respectively. Following ApPDT, cells were prepared for flow cytometry and transmission electron microscopy to unravel the mechanisms of cell death. Our results showed the lowest MB concentration (50 μM) and the lowest optical power (100 mW) promoted the ...Continue Reading

Citations

Dec 22, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Carla Cardozo Pinto de ArrudaKátia da Silva Calabrese
Jun 24, 2021·Photodiagnosis and Photodynamic Therapy·Luciana Maria Cortez MarcolinoJuliana Ferreira Strixino
Dec 14, 2021·Photodermatology, Photoimmunology & Photomedicine·Sercin Ozlem-CaliskanSema Ertug

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