Cell density-dependent gene contributes to efficient seed colonization by Pseudomonas putida KT2440

Applied and Environmental Microbiology
M Espinosa-Urgel, J L Ramos

Abstract

We have characterized the expression pattern of a gene, ddcA, involved in initial colonization of corn seeds by Pseudomonas putida KT2440. The ddcA gene codes for a putative membrane polypeptide belonging to a family of conserved proteins of unknown function. Members of this family are widespread among prokaryotes and include the products of a Salmonella enterica serovar Typhimurium gene expressed during invasion of macrophages and psiE, an Escherichia coli phosphate starvation-inducible gene. Although its specific role is undetermined, the presence of ddcA in multicopy restored the seed adhesion capacity of a KT2440 ddcA mutant. Expression of ddcA is growth phase regulated, being maximal at the beginning of stationary phase. It is independent of RpoS, nutrient depletion, or phosphate starvation, and it is not the result of changes in the medium pH during growth. Expression of ddcA is directly dependent on cell density, being also stimulated by the addition of conditioned medium and of seed exudates. This is the first evidence suggesting the existence of a quorum-sensing system in P. putida KT2440. The potential implication of such a signaling process in seed adhesion and colonization by the bacterium is discussed.

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Citations

Jul 31, 2012·Applied and Environmental Microbiology·Jose M QuesadaManuel Espinosa-Urgel
Sep 30, 2008·Journal of Bacteriology·Regina Fernández-PiñarManuel Espinosa-Urgel
Aug 6, 2008·Annual Review of Phytopathology·Heather L Tyler, Eric W Triplett
Dec 22, 2009·BMC Microbiology·Tanja PiekarskiDieter Jahn
Sep 6, 2007·Genome Biology·Miguel A MatillaMaría Isabel Ramos-González
Jul 12, 2011·Research in Microbiology·Regina Fernández-PiñarManuel Espinosa-Urgel
Apr 4, 2008·Plant Biotechnology Journal·Jorge BarriusoF Javier Gutiérrez Mañero
Jun 15, 2007·Environmental Microbiology·Miguel A MatillaMaría Isabel Ramos-González
Apr 6, 2006·Environmental Microbiology·María Antonia MolinaManuel Espinosa-Urgel
Mar 1, 2008·Microbial Biotechnology·Amalia RocaJuan L Ramos
Jan 1, 2009·Microbial Biotechnology·M Antonia Molina-HenaresEstrella Duque
Jun 14, 2013·Environmental Microbiology Reports·Regina Fernández-PiñarMiguel Cámara
Mar 7, 2014·Environmental Microbiology·Mustafa FazliTim Tolker-Nielsen
Jan 25, 2011·Microbial Biotechnology·Craig DanielsJuan L Ramos
Jun 1, 2010·Environmental Microbiology Reports·Miguel A MatillaMaría Isabel Ramos-González
Jun 1, 2010·Environmental Microbiology Reports·José Juan Rodríguez-HervaJuan-Luis Ramos
Oct 16, 2015·Environmental Microbiology·David Martín-MoraTino Krell
Apr 30, 2015·Molecular Microbiology·Jose Antonio Reyes-DariasTino Krell
Sep 12, 2008·FEMS Microbiology Letters·Fátima Yousef-CoronadoManuel Espinosa-Urgel
Aug 5, 2016·Frontiers in Microbiology·María Isabel Ramos-GonzálezManuel Espinosa-Urgel
Dec 15, 2015·Environmental Microbiology·Andrés Corral-LugoTino Krell
Jun 7, 2015·Applied and Environmental Microbiology·Vanina GarcíaTino Krell
Feb 3, 2005·Environmental Microbiology·María Antonia MolinaManuel Espinosa-Urgel
Jun 10, 2011·Journal of Basic Microbiology·Manoharan ShankarParamasamy Gunasekaran
Aug 15, 2020·Environmental Microbiology·María Victoria Berlanga-ClaveroDiego Romero
Apr 25, 2017·Current Opinion in Microbiology·Ashley ShadeMatthieu Barret
Mar 1, 2019·G3 : Genes - Genomes - Genetics·Ramamoorthy SivakumarJeyaprakash Rajendhran
Sep 14, 2021·Frontiers in Bioengineering and Biotechnology·Yidong LiuCuiqing Ma

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