Cell density regulates crypticity of GM3 ganglioside on human glioma cells

Experimental Cell Research
K TatewakiK Hashi

Abstract

Human glioma cell line KG-1C contains GM3 ganglioside as its sole glycolipid. The degree of M2590 antibody binding to GM3 was found to be regulated by the cell density; the percentage of positive cells in FACS analysis decreased from approximately 20% to close to none as the cells increased their density from sparse to confluent. The contents of GM3 with different cell densities were consistent, being more than 0.4 micromol/g of the cellular weight, which was high enough to be recognized by the antibody. Trypsin treatment of the cells did not increase antibody reactivity. The extracted GM3 retained its antigenicity, being intensely stained with M2590 on a TLC plate; there was no change in chromatographic mobility either, indicating no modification of its chemical structure. The fluorescent microscope disclosed scattered dot-like staining of GM3, particularly at the periphery of the cells. We were able to expose cryptic GM3 fully within 12 h by dispersion of the cells to a sparse density. Surface labeling of GM3 with the use of limited sodium periodate oxidation of sialylated residue equally labeled GM3 either from the confluent cells or the sparse cells. Disassembly of actin filaments with cytochalasin B (10 microM) partially e...Continue Reading

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Citations

Feb 13, 2016·Molecular Neurobiology·Sandro SonninoMassimo Aureli
Sep 29, 2011·Chemistry and Physics of Lipids·Laura CantùAlessandro Prinetti
Dec 2, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·N KovacicA Marusic
Jul 23, 2005·Journal of Neurovirology·Raquel Bello-MoralesJosé Antonio López-Guerrero

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