Cell density regulates neutrophil IL-8 synthesis: role of IL-1 receptor antagonist and soluble TNF receptors

The Journal of Immunology : Official Journal of the American Association of Immunologists
K HattarU Sibelius

Abstract

Although cytokine synthesis in polymorphonuclear leukocytes (PMN) was shown to be modulated by soluble mediators, the impact of microenvironmental conditions has not been elucidated. In this study, we investigated the effect of cell density on cytokine release from human neutrophils. PMN were cultured at various cell densities (10 x 10(6) PMN/ml; 60 x 10(6) PMN/ml), and LPS-induced release of cytokines was quantified by ELISA technique. Upon an increase in PMN density, secretion of the CXC chemokine IL-8 was progressively reduced. This effect was paralleled by a decrease in IL-8 mRNA. In contrast, TNF-alpha and IL-1beta rose proportionally with increasing cell density. The inhibition of IL-8 secretion was reproduced by conditioned media of PMN at high cell density, but was not affected by blocking beta(2) integrin-dependent adhesion. When analyzing the supernatant of LPS-challenged neutrophils, large amounts of soluble TNFRs p55 and p75 (sTNFRI, sTNFRII), and IL-1R antagonist (IL-1RA), rising constantly with the cell density, were detected. Interestingly, combined blocking of the bioactivities of these mediators completely restored neutrophil IL-8 secretion at high cell densities, with the anti-IL-1RA Ab being the more potent a...Continue Reading

References

Apr 1, 1991·The Journal of Experimental Medicine·H R AlexanderJ A Norton
Feb 9, 1989·The New England Journal of Medicine·S J Weiss
May 1, 1988·The Journal of Experimental Medicine·P PeveriM Baggiolini
Dec 1, 1994·Journal of Leukocyte Biology·J A Smith
Mar 26, 1998·Biochemical and Biophysical Research Communications·F Björnberg, M Lantz
Apr 29, 1998·Nature·M Baggiolini
May 23, 1998·Annual Review of Immunology·W P ArendC Gabay
Jun 10, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M K TophamG A Zimmerman
Aug 15, 1998·Annals of the New York Academy of Sciences·M A CassatellaM P Russo
Nov 11, 1998·Nature Medicine·L FinkR M Bohle
Jul 10, 1999·Advances in Immunology·M A Cassatella
Jan 1, 1936·The Journal of Clinical Investigation·O H Robertson, C G Uhley

❮ Previous
Next ❯

Citations

Mar 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Brian EndlichThomas A Hamilton
Feb 27, 2007·Journal of Leukocyte Biology·Filip KukulskiJean Sévigny
Sep 14, 2013·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Hui-Ying TungYufeng Zhou
Dec 22, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Andrew J ThorleyTeresa D Tetley
Feb 3, 2016·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Jennifer M CassanoLisa A Fortier
Nov 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Eva García-RamalloNuria Godessart
Aug 23, 2006·Oral Microbiology and Immunology·A LukićM Colić
Sep 23, 2008·British Journal of Pharmacology·O GripA Bredberg
Jul 13, 2016·Blood·Valérie MonceauxBenoit S Marteyn
Jan 14, 2020·ACS Sensors·Roberto Rodriguez-MoncayoJose Luis Garcia-Cordero

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.