Cell division number regulates IgG1 and IgE switching of B cells following stimulation by CD40 ligand and IL-4

European Journal of Immunology
Jhagvaral HasboldPhilip D Hodgkin

Abstract

CD40 ligand (CD40L) and IL-4 are sufficient to induce resting murine B cells to divide and switch isotypes from IgM and IgD to IgG1 and IgE. Tracking of cell division following (5- and 6) carboxyfluorescein diacetate succinimidyl ester (CFSE) labeling revealed that B cells expressed IgG1 after three cell divisions, and IgE after five. The probability of isotype switching at each division was independent of both time after stimulation and of the dose of CD40L. IL-4 concentration regulated the number of divisions that preceded isotype switching. Loss of surface IgM and IgD was also related to cell division and appeared to be differentially regulated. B cell proliferation was typically asynchronous with the proportion of cells in consecutive divisions being markedly affected by the concentration of CD40L and IL-4. Simultaneous (5-bromo)-2'-deoxyuridine labeling and CFSE staining revealed that B cells in each division cycle were dividing at the same rate. Therefore, division cycle asynchrony resulted from dose-dependent variation in the time taken to enter the first division cycle. These results suggest that T-dependent B cell expansion is linked to predictable functional changes that may, in part, explain why IgE is produced in re...Continue Reading

References

May 7, 1992·Nature·R J ArmitageC R Maliszewski
Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·R J NoelleA Aruffo
Jul 1, 1992·European Journal of Immunology·G SiebenkottenA Radbruch
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·K YoshidaH Sakano
Jan 1, 1989·Advances in Immunology·E S VitettaV M Sanders
Sep 1, 1989·Scandinavian Journal of Immunology·H B MoonU Persson
Jan 1, 1988·The Journal of Experimental Medicine·C M SnapperW E Paul
Jan 1, 1987·Annual Review of Immunology·W E Paul, J Ohara
Aug 1, 1984·The Journal of Experimental Medicine·D Yuan, P W Tucker
Jun 22, 1984·Science·D A Cantrell, K A Smith
Feb 1, 1995·Current Opinion in Immunology·P D Hodgkin, A Basten
May 2, 1994·Journal of Immunological Methods·A B Lyons, C R Parish
Jul 1, 1993·The Journal of Experimental Medicine·M G McHeyzer-WilliamsG J Nossal
Jan 1, 1993·Immunology Today·C M Snapper, J J Mond
Jan 1, 1993·Annual Review of Immunology·D C Parker
Jul 1, 1996·The Journal of Experimental Medicine·P D HodgkinA B Lyons
Jan 1, 1997·International Reviews of Immunology·P D HodgkinJ Hasbold

❮ Previous
Next ❯

Citations

Apr 22, 2009·Bulletin of Mathematical Biology·Ha Youn LeeAlan S Perelson
Oct 18, 2000·Journal of Immunological Methods·P Upadhyay, S Bhaskar
Feb 28, 2001·Journal of Immunological Methods·M E SheehyD F Nixon
Jan 25, 2003·Veterinary Immunology and Immunopathology·A CrawleyB N Wilkie
Jun 6, 2003·Cytokine & Growth Factor Reviews·Gail A Bishop, Bruce S Hostager
Oct 28, 2009·Immunology and Cell Biology·Timothy E SchlubMiles P Davenport
Jun 13, 2006·Nature Immunology·Ulf KleinRiccardo Dalla-Favera
Aug 3, 2002·Immunology·Dania Rabah, Daniel H Conrad
Nov 3, 1998·Immunology and Cell Biology·P D HodgkinJ Hasbold
Nov 26, 1999·Immunology and Cell Biology·B Fazekas de St GrothP Bertolino
Nov 26, 1999·Immunology and Cell Biology·C R Parish
Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Masaki KashiwadaPaul B Rothman
Apr 19, 2000·The Journal of Experimental Medicine·A ShanmugamA L Kenter
Mar 12, 2011·International Immunology·Haifa H JabaraRaif S Geha
Sep 8, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·K Nadira De AbrewRussell S Thomas
Jun 27, 2008·Blood·Danielle T AveryStuart G Tangye
Apr 19, 2011·Allergy, Asthma, and Clinical Immunology : Official Journal of the Canadian Society of Allergy and Clinical Immunology·Aito Ueno-YamanouchiJan Storek
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·A V Gett, P D Hodgkin
Mar 10, 2001·The Journal of Clinical Investigation·T ChitnisS J Khoury
Aug 12, 2005·Proceedings of the National Academy of Sciences of the United States of America·Qiuyan Chen, A Catharine Ross
Sep 6, 2005·Proceedings of the National Academy of Sciences of the United States of America·James S RushDavid G Schatz
Mar 29, 2008·Annual Review of Immunology·Janet StavnezerCarol E Schrader
Jan 12, 2013·Genes & Development·Celia KeimUttiya Basu
Nov 30, 2016·Scientific Reports·Youming ZhangMiriam F Moffatt
Jul 6, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·D J KasprowiczV M Sanders
Jul 6, 2000·Cytometry·J Hasbold, P D Hodgkin
Oct 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·N SchrantzA Vazquez
Oct 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·K HorikawaK Takatsu
Mar 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·James S RushPhilip D Hodgkin
Dec 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Stuart G TangyePhilip D Hodgkin
Jan 9, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Stuart G TangyePhilip D Hodgkin
Jul 24, 2004·Immunology·Stuart G Tangye, Philip D Hodgkin
Sep 24, 2005·European Journal of Immunology·Elissa K DeenickPhilip D Hodgkin
Jun 10, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yusuke OhkuboTakeshi Tokuhisa

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.