Cell-electronic sensing of particle-induced cellular responses

The Analyst
Li HuangXing-Fang Li

Abstract

We report a new technique for the continuous and real-time measurement of microparticle-induced cellular responses using a real-time cell-electronic sensing (RT-CES) technology. The method involves the use of microelectrode-embedded microwells seeded with one of two lung cancer carcinoma cell lines (A549 and SK-MES-1), allowing for continuous measurements of impedance. The change in impedance that is automatically converted to the cell index is linearly correlated with the numbers of the seeding cells during the log phase, providing quantitative measurement of cytotoxicity. After 24 h of initial incubation in 96 microwells, the cultures are treated with microparticles, and changes in the cell index are monitored in real time. Multiple data, including dose response curves, IC(50) (a concentration inhibiting 50% cell growth), and cell-specific and particulate-specific cell responses, are obtained from a single set of experiments. SK-MES-1 cells consistently showed more severe effects and lower IC(50) values than A549 cells when they were treated with quartz particle suspensions. The different effects detected using the RT-CES technique were related to morphological change and apoptosis, supported by the scanning electronic micros...Continue Reading

References

May 15, 1989·Analytical Biochemistry·J A Cook, J B Mitchell
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·I Giaever, C R Keese
Jun 1, 1994·Experimental Cell Research·Z PeiD E Wilson
Sep 15, 2001·Annals of Biomedical Engineering·N DePaolaP Vincent
Oct 10, 2002·The Journal of Allergy and Clinical Immunology·Ajay B AntonyKamal A Mohammed
Feb 25, 2003·Occupational and Environmental Medicine·D BuchananC A Soutar
May 28, 2004·Journal of Clinical Immunology·Gail Ramirez-IcazaVeena B Antony
Sep 11, 2004·Assay and Drug Development Technologies·Kelli SollyWei Zheng
Feb 22, 2005·Chemical Research in Toxicology·James Zan XingXiao Xu
Sep 16, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Tamara Musafia-JeknicWilliam M Baird
Feb 17, 2006·Toxicology in Vitro : an International Journal Published in Association with BIBRA·James Z XingLi Xie
Nov 23, 2006·Assay and Drug Development Technologies·Josephine M AtienzaYama A Abassi
Aug 22, 2007·The Analyst·Fareid Asphahani, Miqin Zhang

❮ Previous
Next ❯

Citations

Nov 8, 2011·Chemical Research in Toxicology·Joanna M SeiffertDamian Marshall
Dec 24, 2011·Assay and Drug Development Technologies·Timo WeilandSascha Venturelli
Oct 10, 2013·Particle and Fibre Toxicology·Melissa A VettenMary Gulumian
Apr 14, 2012·The Analyst·Fareid AsphahaniMiqin Zhang
Dec 22, 2009·Integrative Biology : Quantitative Biosciences From Nano to Macro·Daynene M VykoukalJody Vykoukal
Aug 21, 2012·Cytotherapy·Dominic ClarkeJean Stanton
Sep 11, 2010·Biochemical and Biophysical Research Communications·Peter T WitkowskiAndreas Nitsche
Apr 29, 2008·Analytica Chimica Acta·Jessica M BoydXing-Fang Li
Feb 16, 2013·Journal of Biomedical Materials Research. Part a·Sandra N GarciaAmy McNulty
Nov 21, 2015·Chemistry, an Asian Journal·Jian Yin, Peng Miao
Jul 17, 2020·International Journal of Toxicology·Charlene AndraosMary Gulumian
Jul 5, 2017·Experimental and Therapeutic Medicine·Ke PengFu-Hai Ji

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biosensors for Cancer Detection

Biosensors are devices that are designed to detect a specific biological analyte by essentially converting a biological entity (ie, protein, DNA, RNA) into an electrical signal that can be detected and analyzed. The use of biosensors in cancer detection and monitoring holds vast potential. Biosensors can be designed to detect emerging cancer biomarkers and to determine drug effectiveness at various target sites. Biosensor technology has the potential to provide fast and accurate detection, reliable imaging of cancer cells, and monitoring of angiogenesis and cancer metastasis, and the ability to determine the effectiveness of anticancer chemotherapy agents.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis