Cell-engineered human elastic chondrocytes regenerate natural scaffold in vitro and neocartilage with neoperichondrium in the human body post-transplantation.

Tissue Engineering. Part a
Hiroko YanagaKatsu Yanaga

Abstract

We have developed a unique method that allows us to culture large volumes of chondrocyte expansion from a small piece of human elastic cartilage. The characteristic features of our culturing method are that fibroblast growth factor-2 (FGF2), which promotes proliferation of elastic chondrocytes, is added to a culture medium, and that cell-engineering techniques are adopted in the multilayered culture system that we have developed. We have subsequently discovered that once multilayered chondrocytes are transplanted into a human body, differentiation induction that makes use of surrounding tissue occurs in situ, and a large cartilage block is obtained through cartinogenesis and matrix formation. We have named this method two-stage transplantation. We have clinically applied this transplantation method to the congenital ear defect, microtia, and reported successful ear reconstruction. In our present study, we demonstrated that when FGF2 was added to elastic chondrocytes, the cell count increased and the level of hyaluronic acid, which is a major extracellular matrix (ECM) component, increased. We also demonstrated that these biochemical changes are reflected in the morphology, with the elastic chondrocytes themselves producing a ma...Continue Reading

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Citations

Jan 21, 2014·International Journal of Pediatric Otorhinolaryngology·Mark ShastiWilliam J Landis
Oct 16, 2013·Tissue Engineering. Part a·Renata Giardini-RosaStephen D Waldman
Feb 24, 2015·Journal of Cranio-maxillo-facial Surgery : Official Publication of the European Association for Cranio-Maxillo-Facial Surgery·E J BosP van Zuijlen
Apr 30, 2016·Connective Tissue Research·Thomas J KeanJames E Dennis
Jun 9, 2016·Burns : Journal of the International Society for Burn Injuries·E J BosP P van Zuijlen
Sep 3, 2016·Histochemistry and Cell Biology·Laura García-MartínezVíctor Carriel
Nov 22, 2016·The Laryngoscope·Pedram AkbariWalid A Farhat
Nov 11, 2016·Tissue Engineering. Part C, Methods·Pedram AkbariWalid A Farhat
Jun 13, 2015·Cartilage·Alan TsengCathryn A Sundback
Feb 18, 2016·Cell Transplantation·Shintaro KagimotoHideki Taniguchi
Mar 15, 2018·Annals of Plastic Surgery·Jaime L BernsteinJason A Spector
Nov 16, 2017·Journal of Tissue Engineering and Regenerative Medicine·Aijuan HeGuangdong Zhou
Jan 31, 2019·The Laryngoscope·Loraine L Y ChiuStephen D Waldman
Aug 2, 2019·The Journal of Craniofacial Surgery·Mimi R BorrelliHermann Peter Lorenz
Aug 28, 2020·Frontiers in Cell and Developmental Biology·Eleonora ZucchelliPatrizia Ferretti
May 11, 2021·Tissue Engineering. Part B, Reviews·David GvaramiaNicole Rotter

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Methods Mentioned

BETA
scanning electron microscopy

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