Cell-free 3D scaffold with two-stage delivery of miRNA-26a to regenerate critical-sized bone defects

Nature Communications
Xiaojin ZhangPeter X Ma

Abstract

MicroRNAs (miRNAs) are being developed to enhance tissue regeneration. Here we show that a hyperbranched polymer with high miRNA-binding affinity and negligible cytotoxicity can self-assemble into nano-sized polyplexes with a 'double-shell' miRNA distribution and high transfection efficiency. These polyplexes are encapsulated in biodegradable microspheres to enable controllable two-stage (polyplexes and miRNA) delivery. The microspheres are attached to cell-free nanofibrous polymer scaffolds that spatially control the release of miR-26a. This technology is used to regenerate critical-sized bone defects in osteoporotic mice by targeting Gsk-3β to activate the osteoblastic activity of endogenous stem cells, thus addressing a critical challenge in regenerative medicine of achieving cell-free scaffold-based miRNA therapy for tissue engineering.

References

Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M R UristA Lietze
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R W MaloneI M Verma
Mar 22, 2001·Advanced Drug Delivery Reviews·C W Pouton, L W Seymour
Oct 21, 2003·Journal of Biomedical Materials Research. Part a·Kyung Mi WooPeter X Ma
Oct 31, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Georges RawadiSergio Roman-Roman
May 4, 2004·Methods : a Companion to Methods in Enzymology·Brian DalbyValentina C Ciccarone
Oct 19, 2004·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·O Johnell, J A Kanis
Jul 30, 2005·Nature Reviews. Drug Discovery·Daniel W PackPatrick S Stayton
Nov 1, 2005·Nature·Jan KrützfeldtMarkus Stoffel
Mar 28, 2006·Biomaterials·Victor J ChenPeter X Ma
Apr 26, 2006·Journal of Biomedical Materials Research. Part a·Guobao Wei, Peter X Ma
Jun 7, 2006·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Nalini H KulkarniYanfei L Ma
Jan 19, 2007·Nature Reviews. Genetics·Kevin Chen, Nikolaus Rajewsky
Feb 6, 2007·Angewandte Chemie·Michał R RadowskiRainer Haag
Apr 24, 2007·Advanced Drug Delivery Reviews·Lars Aagaard, John J Rossi
Nov 30, 2007·Advanced Drug Delivery Reviews·Peter X Ma
Jan 17, 2008·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Ettore LuziMaria Luisa Brandi
Feb 19, 2008·Bone·Lynda F Bonewald, Mark L Johnson
Jul 26, 2008·Nature Nanotechnology·Dan PeerRobert Langer
Dec 5, 2008·Chemical Reviews·Meredith A Mintzer, Eric E Simanek
Nov 13, 2009·Gene Therapy·S Nayak, R W Herzog
May 15, 2010·Science·S Hani Najafi-ShoushtariAnders M Näär
May 15, 2010·Science·Katey J RaynerCarlos Fernández-Hernando
Jun 23, 2010·Proceedings of the National Academy of Sciences of the United States of America·Tyler J MarquartAngel Baldán
Nov 9, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yanan YueChi Wu
Jan 31, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Guang-Yuh ChiouShih-Hwa Chiou
Feb 3, 2012·ACS Nano·Kathrin FuhrmannJean-Christophe Leroux
Feb 4, 2012·Circulation Research·Eva van RooijArthur A Levin
Dec 12, 2012·Nature Medicine·Xiaogang WangYingxian Li
Aug 24, 2013·Science·Inder M Verma

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Citations

Feb 12, 2017·Materials Science & Engineering. C, Materials for Biological Applications·Fernando José Costa BaratélaAlvaro Antonio Alencar de Queiroz
Feb 9, 2018·Tissue Engineering. Part a·Eric N JamesDavid L Kaplan
Nov 13, 2018·Macromolecular Rapid Communications·Yu DaiXiaojin Zhang
Sep 23, 2018·Gene Therapy·Ruying ChenJames D Bryers
Feb 5, 2019·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Joyce Bj van MeursJose A Riancho
Jan 24, 2019·Journal of Biomedical Materials Research. Part a·Kathryn L WoffordKara L Spiller
Dec 6, 2017·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Pierre J Marie, Martine Cohen-Solal
Mar 14, 2019·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Michael Hadjiargyrou, David E Komatsu
Mar 25, 2019·Journal of Biomedical Materials Research. Part a·Yan SunXiao-Hong Li
Jun 22, 2019·Expert Opinion on Drug Delivery·Ana Cláudia LimaNuno M Neves
May 3, 2019·Drug Delivery and Translational Research·Eunmi BanAeri Kim
Jul 15, 2016·Science Translational Medicine·Yu MiyazakiChristopher M Gomez
Oct 23, 2019·Cellular and Molecular Life Sciences : CMLS·Sanda BocaIoana Berindan-Neagoe
Feb 20, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yixuan ChenYoushui Gao
May 6, 2020·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Timothy Cuneo, Haifeng Gao
Jul 11, 2020·Journal of Biomaterials Science. Polymer Edition·Liangrui ChenXueqi Gan
Sep 12, 2020·Journal of Tissue Engineering and Regenerative Medicine·Reza Eivazzadeh-KeihanMichael R Hamblin
Oct 5, 2019·Journal of Nanobiotechnology·Mengfei YuLingqing Dong
Jul 15, 2016·Journal of Materials Science. Materials in Medicine·Mahya SadeghiArash Khojasteh
Sep 6, 2018·Bone Research·Ming DangPeter X Ma
Dec 20, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Li LiuYan-Qing Guan
Jul 31, 2018·Frontiers in Bioengineering and Biotechnology·Celeste PiottoMikaël M Martino
Oct 27, 2017·Advanced Healthcare Materials·Caroline M CurtinFergal J O'Brien
Jun 28, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Minh Khanh NguyenEben Alsberg
Feb 3, 2018·International Journal of Molecular Medicine·Maria Teresa ValentiMonica Mottes
Jul 2, 2019·International Journal of Molecular Medicine·Xiaoyan YuanJie Long
Aug 20, 2019·International Journal of Oral Science·Chenxi ZhengYan Jin
Apr 10, 2019·International Journal of Endocrinology·Xi SunXianghang Luo
Nov 14, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Chuanqing Lan, Shulin Zhao
Jun 2, 2020·Frontiers in Bioengineering and Biotechnology·Tingting YuBing Han
Jul 11, 2020·Biomedical Materials·Satheesh ElangovanAliasger K Salem
Oct 3, 2020·Bioactive Materials·Fengqing ShangYan Jin

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Methods Mentioned

BETA
transfection
antisense oligoribonucleotides
transmission electron microscopy
dynamic light scattering
electrophoresis
transfect
scanning electron microscopy
PCR
fluorescence microscopy

Software Mentioned

Image J
pro plus
Image

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