Cell-free amino acid-incorporating system from Pseudomonas indigofera.

Journal of Bacteriology
T Shiio, B A MCFADDEN

Abstract

Shiio, Tsuru (Washington State University, Pullman), and Bruce A. McFadden. Cell-free amino acid-incorporating system from Pseudomonas indigofera. J. Bacteriol. 90:978-983. 1965.-A cell-free preparation from Pseudomonas indigofera incorporated C(14)-phenylalanine and C(14)-leucine into a product which was insoluble in hot trichloroacetic acid. The phenylalanine incorporation process, which had a temperature optimum of 30 C and a pH optimum of 7.6, had many characteristics of protein synthesis. The process depended upon both "ribosomes" and supernatant fraction from centrifugation at 105,000 x g. Incorporation required adenosine triphosphate, apparently depended upon guanosine triphosphate, and was inhibited by chloramphenicol, puromycin, actinomycin, ribonuclease, and deoxyribonuclease. Leucine incorporation was also studied and had many similar characteristics. C(14)-phenylalanine uptake was stimulated by sRNA or polyuridylic acid, and together these substances had a synergistic effect upon stimulation. The incorporation of C(14)-phenylalanine into a product which was precipitated by antiserum to crystalline isocitrate lyase was also observed.

References

Feb 1, 1964·The Biochemical Journal·D H BishopB Nisman
Jul 15, 1961·Proceedings of the National Academy of Sciences of the United States of America·G VON EHRENSTEIN, F LIPMANN
Sep 10, 1963·Biochemical and Biophysical Research Communications·H R WHITELEY, M OISHI
Sep 1, 1963·Biochemistry·R K BRETTHAUERR M BOCK
Dec 1, 1959·Proceedings of the National Academy of Sciences of the United States of America·M B Yarmolinsky, G L Haba

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Citations

Jul 23, 1968·Biochimica Et Biophysica Acta·T TaniJ Shishiyama

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