Cell-free extracts of Propionibacterium acnes stimulate cytokine production through activation of p38 MAPK and Toll-like receptor in SZ95 sebocytes

Life Sciences
Yu-Chun HuangHan-Chi Hsu

Abstract

Propionibacterium acnes has been considered to influence the acne lesions. The present study intended to elucidate the underlying signaling pathways of P. acnes in human sebaceous gland cells relative to the generation of proinflammatory cytokines. Cell-free extracts of P. acnes under stationary growth phase were co-incubated with human immortalized SZ95 sebocytes. Then, cell-free P. acnes extracts-induced cytokine expression was evaluated by measuring mRNA and protein levels using quantitative RT-PCR and ELISA. Changes of phosphorylated cell signaling proteins and transcription factors were measured by Western blots and Milliplex assay. The interactive molecular mechanisms of P. acnes and sebocytes were examined through use of shRNA and the specific inhibitors of signaling pathways. Cell-free extracts of P. acnes significantly stimulated secretion of interleukin (IL)-8 and IL-6 in SZ95 sebocytes. The degradation of IκB-α and increased phosphorylation of IκB-α, p38 mitogen activated protein kinase (MAPK), CREB, and STAT3 were demonstrated. Quantitative RT-PCR measurements revealed that gene expression of IL-8 and Toll-like receptor 2 (TLR2) was enhanced by cell-free extracts of P. acnes. In addition, the NF-κB inhibitor BMS3455...Continue Reading

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Citations

Jul 13, 2019·Journal of the European Academy of Dermatology and Venereology : JEADV·M-A DagnelieB Dréno
Dec 22, 2019·Experimental Dermatology·Gwendal JosseEtienne Gontier
Jun 20, 2017·Expert Opinion on Investigational Drugs·Christos C ZouboulisHarald P M Gollnick
Nov 14, 2017·Skin Pharmacology and Physiology·Eleonora BassinoLuca Munaron
Aug 12, 2020·Experimental Dermatology·Christos C Zouboulis
Jul 13, 2021·Experimental Dermatology·Mahendran Chinnappan, Tamia A Harris-Tryon

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