Cell free mitochondrial DNA in serum and milk associated with bovine mastitis: a pilot study

Veterinary Research Communications
Geeta Devi LeishangthemAmarjit Singh

Abstract

Mastitis is inflammation of mammary gland affecting all the species of domestic animals. Fragments of the mitochondrial genome released from dying cells are considered surrogate markers of mitochondrial injury. We hypothesized that bovine mastitis would be associated with increased cell free mitochondrial DNA (mtDNA) content in serum and milk. Milk and serum samples were collected from sub-clinical mastitic and normal animals. Mastitis was confirmed by California mastitis test and bacterial isolation. Oxidative stress, nitric oxide and inflammatory cytokines were also estimated. Real time polymerase chain reaction was conducted in serum and milk from sub-clinical mastitic animals and compared with healthy animals targeting the mtDNA genes cytochrome b. Mastitis animals showed higher oxidative stress markers and nitric oxide along with higher level of inflammatory cytokines. Cell free mtDNA was significantly higher in serum and milk of mastitic animals comparing to that of healthy control. The higher cell free relative mtDNA content in mastitis animals indicates injury to the mammary epithelial cells and thereby releasing the mtDNA in the milk and blood. This mtDNA may be a bio-marker of oxidative stress and tissue injury in bov...Continue Reading

References

Feb 15, 1991·Clinica Chimica Acta; International Journal of Clinical Chemistry·L Góth
Jun 1, 1984·Toxicology Letters· Shafiq-Ur-Rehman
Jan 12, 2000·Journal of Dairy Science·L BouchardP Lacasse
Aug 30, 2001·Cell Death and Differentiation·E LongX Zhao
Dec 5, 2002·Journal of Mammary Gland Biology and Neoplasia·Lorraine M Sordillo, Katie L Streicher
Apr 1, 2004·Journal of Agricultural and Food Chemistry·Stefania Cesa
Apr 21, 2004·Immunology·Terri N Ellis, Blaine L Beaman
Dec 8, 2004·Cellular and Molecular Life Sciences : CMLS·M PatrushevA Gaziev
Nov 23, 2006·Antioxidants & Redox Signaling·Stefan W RyterAugustine M K Choi
Jun 15, 2007·Toxicologic Pathology·Susan Elmore
Jun 15, 2007·Infection and Immunity·Johann S BraunJoerg R Weber
Sep 6, 2007·Journal of Animal Science·X Zhao, P Lacasse
Dec 17, 2008·International Immunopharmacology·Beibei GuSixiang Zou
Jun 6, 2009·American Journal of Veterinary Research·Sarah A WagnerMichael D Apley
Oct 3, 2009·Current Opinion in Pharmacology·Darcy L Johannsen, Eric Ravussin
Jul 20, 2010·Cell Host & Microbe·Mohamed Lamkanfi, Vishva M Dixit
Jan 12, 2011·Annual Review of Immunology·Roman BarbalatGregory M Barton
Aug 13, 2011·The Journal of Biological Chemistry·Toren Finkel
Oct 23, 2012·Mitochondrion·Afshan N Malik, Anna Czajka
Aug 29, 2013·International Journal of Biological Sciences·Neelesh Sharma, Dong Kee Jeong
Feb 27, 2014·European Heart Journal·Camilla Ferreira WenceslauUNKNOWN Working Group on DAMPs in Cardiovascular Disease
Sep 23, 2014·Nature Reviews. Molecular Cell Biology·Prashant Mishra, David C Chan
Dec 20, 2014·Journal of Mammary Gland Biology and Neoplasia·John R MiddletonK Larry Smith
Feb 25, 2015·Biochemical Pharmacology·Elodie LobetThierry Arnould
Jun 13, 2015·Antioxidants & Redox Signaling·Kiichi NakahiraAugustine M K Choi
Aug 26, 2015·Animal Health Research Reviews·Valerie E RymanLorraine M Sordillo
Sep 12, 2017·Animal Science Journal = Nihon Chikusan Gakkaihō·Yuya NagasawaTomohito Hayashi

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Citations

Jul 29, 2019·Research in Veterinary Science·Svetlana NedićSunčica Borozan

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Methods Mentioned

BETA
ELISA
MDA
electrophoresis
PCR

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