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Cell-Free Mixing of Escherichia coli Crude Extracts to Prototype and Rationally Engineer High-Titer Mevalonate Synthesis

ACS Synthetic Biology

Dec 16, 2016

Quentin Merle DudleyMichael Alexander Spencer Jewett

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Abstract

Cell-free metabolic engineering (CFME) is advancing a powerful paradigm for accelerating the design and synthesis of biosynthetic pathways. However, as most cell-free biomolecule synthesis systems to date use purified enzymes, energy and cofactor balance can be limiting. To address this...read more

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Enzymes, antithrombotic
Landscapes
Enzymes, peripheral vasodilators
Genome, Bacterial
Plant Extracts
Gene Knockout Techniques
CoFactor brand of 5,10-methylenetetrahydrofolate
AT 17
Enzymes, hematological
Genome
25
107
Paper Details
References
  • References73
  • Citations6
12345...

Cell-Free Mixing of Escherichia coli Crude Extracts to Prototype and Rationally Engineer High-Titer Mevalonate Synthesis

ACS Synthetic Biology

Dec 16, 2016

Quentin Merle DudleyMichael Alexander Spencer Jewett

PMID: 27476989

DOI: 10.1021/acssynbio.6b00154

Abstract

Cell-free metabolic engineering (CFME) is advancing a powerful paradigm for accelerating the design and synthesis of biosynthetic pathways. However, as most cell-free biomolecule synthesis systems to date use purified enzymes, energy and cofactor balance can be limiting. To address this...read more

Mentioned in this Paper

Enzymes, antithrombotic
Landscapes
Enzymes, peripheral vasodilators
Genome, Bacterial
Plant Extracts
25
107

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Paper Details
References
  • References73
  • Citations6
12345...

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