Cell-Free Synthesis of a Functional Membrane Transporter into a Tethered Bilayer Lipid Membrane

Langmuir : the ACS Journal of Surfaces and Colloids
Julius L ZielenieckiIngo Köper

Abstract

Eukaryotic cell-free synthesis was used to incorporate the large and complex multispan plant membrane transporter Bot1 in a functional form into a tethered bilayer lipid membrane. The electrical properties of the protein-functionalized tethered bilayer were measured using electrochemical impedance spectroscopy and revealed a pH-dependent transport of borate ions through the protein. The efficacy of the protein synthesis has been evaluated using immunoblot analysis.

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Citations

Apr 19, 2018·The Analyst·Meriem ChadliAgnès Girard-Egrot
Apr 28, 2018·Physical Chemistry Chemical Physics : PCCP·Jakob AnderssonIngo Köper
Oct 16, 2016·Cold Spring Harbor Perspectives in Biology·Jessica G PerezMichael C Jewett
Aug 10, 2019·BMC Biology·Aidan TinafarKeith Pardee
Jul 8, 2021·Trends in Biotechnology·A K JayaramR M Owens
Jul 30, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·J BompardA Girard-Egrot
Sep 7, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Meriem ChadliAgnès Girard-Egrot
Oct 10, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Ahlam AlharbiGunther G Andersson
Sep 14, 2020·Methods : a Companion to Methods in Enzymology·Jakob AnderssonWolfgang Knoll
Oct 23, 2021·ACS Applied Materials & Interfaces·Eider Berganza, Michael Hirtz

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