PMID: 6166470May 1, 1981Paper

Cell-free translation of messenger RNAs from adult and fetal human muscle. Characterization of neosynthesized glycogen phosphorylase, phosphofructokinase and glucose phosphate isomerase

European Journal of Biochemistry
A KahnJ C Dreyfus

Abstract

Using a procedure of ethanol precipitation in concentrated guanidine . HCl solutions followed by chloroform/isoamylic alcohol extraction and washing in 3 M sodium acetate, we isolated high-molecular-weight cellular RNA from human fetal and adult skeletal muscle. About 500 micrograms RNA were obtained/g of fetal muscle and 50 micrograms RNA/g of adult muscle. Both RNA preparations were efficiently translated in a cell-free reticulocyte lysate system and directed synthesis of various polypeptides, one of them of Mr 200,000 probably corresponding to myosin heavy chains. Dodecylsulphate/polyacrylamide gel electrophoretic pattern of polypeptides neosynthesized using either fetal or adult RNA exhibited several differences. Three neosynthesized cytosolic muscle enzymes were purified from the translation mixtures using a micro-method of immunoaffinity chromatography; specificity of the neosynthesized polypeptides purified according to this procedure was checked by immunological competition with the corresponding unlabeled pure muscle enzymes. Successful cell-free translation of RNA from adult skeletal muscle and purification of neosynthesized human enzymes are reported for the first time. These methods, indispensable for further studie...Continue Reading

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Citations

Jan 1, 1986·Journal of Inherited Metabolic Disease·D M Swallow, D A Hopkinson
Jan 1, 1987·Journal of Steroid Biochemistry·J G LehouxD Bellabarba
May 15, 1985·Thrombosis Research·F BellocJ Soria
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·P H RomeoM Goossens
Aug 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·B GrandchampM Goossens
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J Y PicardA Kahn
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·T Lacaze-MasmonteilX Bertagna
Jun 1, 1987·The Journal of Cell Biology·A DuperrayG Marguerie
Jun 11, 1983·Nucleic Acids Research·A HanauerJ L Mandel
Jul 15, 1983·European Journal of Biochemistry·A EisenmannP C Heinrich
Jun 1, 1996·Journal of Cardiovascular Electrophysiology·E SosaF Pilleggi
Mar 1, 1985·The Journal of Clinical Investigation·A MunnichA Kahn
Jun 1, 1988·The Journal of Clinical Investigation·S LyonnetA Munnich
Sep 1, 1990·The Journal of Clinical Investigation·Y de KeyzerX Bertagna
Mar 29, 1982·Biochimica Et Biophysica Acta·J MarieA Kahn
Feb 29, 1984·Biochemical and Biophysical Research Communications·G UzanA Kahn
Dec 16, 1983·Biochemical and Biophysical Research Communications·C BesmondA Kahn
Apr 30, 1984·Biochemical and Biophysical Research Communications·G UzanG Marguerie
May 31, 1985·Biochemical and Biophysical Research Communications·F MarlhensP M Sinet
Feb 1, 1986·Bioscience Reports·M Devchand, M Kapoor
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Aug 1, 1984·Journal of Muscle Research and Cell Motility·G GillespieY Edwards
Nov 18, 1988·Biochimica Et Biophysica Acta·J BonaventureM T Corvol
Sep 1, 1987·Experimental Cell Research·N DenisA Kitzis

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