PMID: 6168290Jun 26, 1981Paper

Cell-free translation of RNA isolated from aldosterone-treated toad bladder mucosal cells

Biochimica Et Biophysica Acta
C P YangW N Scott

Abstract

The stimulation of sodium transport by aldosterone in target tissues requires the synthesis of both mRNA and proteins. Aldosterone-induced mRNA and proteins have been demonstrated in toad urinary bladder and rat kidney. We have isolated total RNA and poly(A)-containing RNA from hormone-treated and untreated toad bladder mucosal cells for translation in a rabbit reticulocyte lysate system. Aldosterone-induced proteins synthesized in this system have physical properties similar to those of aldosterone-induced proteins synthesized in the intact toad bladder.

References

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Citations

Jan 1, 1981·Annals of the New York Academy of Sciences·W N ScottP M Terry

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