Cell fusion activities of Hantaan virus envelope glycoproteins

Journal of Virology
Michiko OginoJ Arikawa

Abstract

Hantaan virus (HTNV)-infected Vero E6 cells undergo cell fusion with both infected and uninfected cells under low-pH conditions. Flow cytometry and fluorescence microscopy of HTNV-infected Vero E6 cells showed that envelope glycoproteins (GPs) were located both on the cell surface and in the cytoplasm. Neutralizing monoclonal antibodies (MAbs) against the G1 and G2 envelope GPs inhibited cell fusion, whereas nonneutralizing MAbs against G1 or G2 and MAbs against the nucleocapsid protein (NP) did not. Transfected Vero E6 cells that expressed GPs but not those that expressed NP fused and formed syncytia. These results indicate that HTNV GPs act as fusogens at the cell surface. No fusion activity was observed either in infected Vero cells that were passaged more than 150 times or in BHK-21 cells, although GPs appeared to localize to the cell surface. This variability in fusion induction suggests the involvement of host cell factors in the process of cell membrane fusion.

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Citations

Apr 12, 2012·Virologica Sinica·Shu-ling ZhaoChang-yong Liang
Oct 16, 2009·Journal of Virology·Jussi HepojokiHilkka Lankinen
Feb 16, 2007·Journal of Virology·Manoj N KrishnanErol Fikrig
May 31, 2013·Journal of Virology·Rajini MudhasaniSina Bavari
Jun 8, 2012·The Journal of Veterinary Medical Science·Vu Dinh LuanJiro Arikawa
Feb 12, 2014·PLoS Pathogens·Josiah PetersenKenneth Briley
Sep 29, 2011·Virus Research·Adam MacneilChristina F Spiropoulou
Jul 14, 2010·Virus Research·Nicolás Cifuentes-MuñozNicole D Tischler
Aug 28, 2015·The Journal of General Virology·Rodrigo AcuñaNicole D Tischler
May 25, 2012·The Journal of General Virology·Jussi HepojokiAntti Vaheri
Oct 27, 2016·PLoS Pathogens·Shmuel WillenskyMoshe Dessau
Oct 18, 2005·The Journal of General Virology·Nicole D TischlerPablo D T Valenzuela
Dec 22, 2006·Clinical and Vaccine Immunology : CVI·Megumi OkumuraJiro Arikawa
Nov 5, 2017·Archives of Virology·Yuji Isegawa, Yoshinobu Okuno
Jul 3, 2009·The Journal of General Virology·Xiaohong ShiRichard M Elliott
Jan 27, 2019·Scientific Reports·Hannah Sabeth SperberRoland Schwarzer

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