PMID: 6988994Jan 1, 1980Paper

Cell fusion-impaired variants of a mouse lymphocytic cell line obtained by single-step selection for polyethylene glycol resistance

Somatic Cell Genetics
J K StadlerJ Hanser


This report describes an effective method for single-step isolation of cell line variants with reduced intercellular plasma membrane fusion ability. Variants, derived from a subline of L5178Y, were obtained by selection for growth in the presence of a toxic level of PEG 6000. These variants occurred after EMS treatment and spontaneously. Further testing revealed a pleiotropic phenotype, fusion impairment and polyethylene glycol resistance, which has been designated PgfR. Variant sublines, in serial subculture for more than 165 days on nonselective medium, retain a stable PgfR phenotype. PgfR variants have an approximate 7-fold reduction in intercellular fusion competence.


Jan 17, 1975·Nature·Q F AhkongJ A Lucy
Jul 1, 1971·Journal of Theoretical Biology·G Poste, A C Allison
Jul 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·J K Stadler, E A Adelberg
Dec 28, 1973·Biochimica Et Biophysica Acta·G Poste, A C Allison


Feb 1, 1986·Journal of Cellular Physiology·W D LiW C Shen

Related Concepts

Cell Fusion Procedure
Clone Cells
Drug Resistance
Ethyl Methanesulfonate
Genetic Techniques
Lymphoid Cells
Variation (Genetics)

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