Cell growth and protein formation on various microcarriers.

Cytotechnology
D KongJ Zhang

Abstract

A large number of microcarriers are commercially available. The capability of cells to successfully proliferate on microcarriers varies with cell lines and media. Choosing the right microcarrier for a particular cell line is more than a choice of a microcarrier. It is part of an integrated process design. A detailed picture of cell growth and product formation will not only be essential in identifying the kind of microcarrier, but also in determining other parts of the process, such as operation mode and media. Our initial screening on thirteen microcarriers showed that cultures on some microcarriers reached a low cell density but high cell-specific productivity, and high density microcarrier cultures have a low specific productivity. The result is a similar product output per unit volume and time for these two types of cultures. An ideal culture system shall have increased volumetric productivity at elevated cell density. This requires the process goal to be incorporated as early as cell line construction and screening. A high output process can then be realized through high density culture.

References

Mar 1, 1992·Enzyme and Microbial Technology·T J Nikolai, W S Hu
Jan 1, 1988·Biotechnology & Genetic Engineering Reviews·K Nilsson

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Citations

Dec 21, 2013·In Vitro Cellular & Developmental Biology. Animal·Duygu Ayyildiz-TamisS Ismet Deliloglu-Gurhan
Dec 12, 2013·Journal of Biomaterials Applications·Jason FengEng San Thian
Jul 1, 2010·Biotechnology and Bioengineering·Michael P StormMelanie J Welham
Jan 1, 2010·Biotechnology Progress·Maria Elisa RodriguesRosário Oliveira
May 16, 2009·Journal of Tissue Engineering and Regenerative Medicine·Sofia PetterssonGunnar Kratz
Aug 9, 2003·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Liguo ZhangYaoting Yu
May 20, 2021·Preparative Biochemistry & Biotechnology·Mohd Azmir ArifinHamzah Mohd Salleh

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