Cell growth rate regulates expression of group B Streptococcus type III capsular polysaccharide.

Infection and Immunity
L C PaolettiK D Johnson

Abstract

The capsular polysaccharide (CPS) of group B streptococci (GBS) is an important virulence factor that also serves to protect cells from nonspecific host defense mechanisms. Expression of CPS by GBS, as with other encapsulated bacterial pathogens, is not constitutive but varies during growth in vitro and in primary cultures isolated from different sites of infection. Despite this understanding, little is known about regulation of this surface-expressed carbohydrate antigen in GBS. Here we report that expression of type III CPS by GBS strain M781 grown in continuous culture with a modified chemically defined medium is regulated by growth rate. Cells in steady state at mass doubling times (tds) of 0.8, 1.4, and 1.6 h expressed an average of sixfold more cell-associated CPS than did cells held at tds of 2.3 and 11 h. Strain M781 grown at a td of 1.4 h repeatedly produced more type III CPS than those held at a td of 11.0 h, even when limited for glucose, pyridoxamine, or thiamine. In our studies, > or = 93% of the total CPS expressed by strain M781 was cell associated. Strain M781 grown at a td of 11.0 h (i.e., lowered CPS expression) was susceptible to in vitro complement-mediated opsonophagocytosis and killing by human peripheral ...Continue Reading

References

May 1, 1978·Journal of Bacteriology·F A MianR C Righelato
Aug 1, 1979·Journal of Bacteriology·W ChesbroR Eifert
Dec 1, 1992·Infection and Immunity·C E RubensG van Belle
Jun 1, 1992·Journal of General Microbiology·I T WibawanF H Pasaribu
Nov 1, 1990·The Journal of Clinical Investigation·M R WesselsH J Jennings
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M R WesselsD L Kasper
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J M MusserR K Selander
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·C E RubensD L Kasper
Jan 1, 1982·Advances in Microbial Physiology·I W Sutherland
Feb 1, 1980·Infection and Immunity·I van de Rijn, R E Kessler
Jun 1, 1980·Infection and Immunity·G B Calandra, R M Cole
Feb 1, 1981·Infection and Immunity·T I DoranS J Mattingly
May 1, 1994·The Journal of Applied Bacteriology·Y MengistuJ R Saunders
Aug 1, 1983·Applied and Environmental Microbiology·D K O'toole

❮ Previous
Next ❯

Citations

Dec 12, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·P HennekeD T Golenbock
Apr 22, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Philipp HennekePatrick Trieu-Cuot
Feb 6, 2009·Emerging Infectious Diseases·Parham SendiAnna Norrby-Teglund
Mar 5, 2009·Future Microbiology·Lakshmi Rajagopal
Apr 1, 2009·Infection and Immunity·Sybille KenzelPhilipp Henneke
May 27, 2011·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·R CretiL Baldassarri
Sep 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Philipp HennekeDouglas T Golenbock
Nov 1, 2001·Proceedings of the National Academy of Sciences of the United States of America·G Malin, L C Paoletti
Nov 26, 2010·Journal of Bacteriology·Brett R HansonMelody N Neely
Feb 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Richard MalleyDouglas T Golenbock
Oct 17, 2008·European Journal of Immunology·Thomas AreschougSiamon Gordon
Feb 24, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sybille KenzelPhilipp Henneke
May 10, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Philipp HennekeDouglas T Golenbock
Jul 18, 2003·Journal of Bacteriology·Lynn E HancockMichael S Gilmore
May 4, 1999·Infection and Immunity·A C WrightM B Sztein
Aug 28, 1999·Journal of Bacteriology·R A RossL C Paoletti
Jan 4, 2001·Microbes and Infection·B Spellerberg
Mar 16, 2019·Microbiology Spectrum·Lawrence C Paoletti, Dennis L Kasper

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.