Cell handling using microstructured membranes

Lab on a Chip
Daniel Irimia, Mehmet Toner

Abstract

Gentle and precise handling of cell suspensions is essential for scientific research and clinical diagnostic applications. Although different techniques for cell analysis at the micro-scale have been proposed, many still require that preliminary sample preparation steps be performed off the chip. Here we present a microstructured membrane as a new microfluidic design concept, enabling the implementation of common sample preparation procedures for suspensions of eukaryotic cells in lab-on-a-chip devices. We demonstrate the novel capabilities for sample preparation procedures by the implementation of metered sampling of nanoliter volumes of whole blood, concentration increase up to three orders of magnitude of sparse cell suspension, and circumferentially uniform, sequential exposure of cells to reagents. We implemented these functions by using microstructured membranes that are pneumatically actuated and allowed to reversibly decouple the flow of fluids and the displacement of eukaryotic cells in suspensions. Furthermore, by integrating multiple structures on the same membrane, complex sequential procedures are possible using a limited number of control steps.

References

Apr 1, 1994·Scandinavian Journal of Immunology·D Stibenz, C Bührer
Feb 13, 2001·Biophysical Journal·J VoldmanM A Schmidt
May 9, 2001·IEEE Transactions on Bio-medical Engineering·I K GlasgowM B Wheeler
Dec 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Carl L HansenStephen R Quake
Oct 23, 2003·Analytical Chemistry·Aaron R WheelerAntoine Daridon
Nov 13, 2003·Electrophoresis·Samuel K Sia, George M Whitesides
Mar 31, 2004·Lab on a Chip·Urban SegerPhilippe Renaud
Jun 5, 2004·Biotechnology Progress·Eric LeclercTeruo Fujii
Oct 16, 2004·Analytical Chemistry·Daniel IrimiaMehmet Toner
Nov 2, 2004·Analytical Chemistry·Palaniappan SethuMehmet Toner
Dec 23, 2004·Biophysical Journal·Adam Rosenthal, Joel Voldman
Mar 8, 2005·Biomolecular Engineering·Nicholas J PanaroPeter Wilding
Jul 12, 2005·Annual Review of Biomedical Engineering·Mehmet Toner, Daniel Irimia
Jul 27, 2005·Proceedings of the National Academy of Sciences of the United States of America·Greg C Randall, Patrick S Doyle
Aug 2, 2005·Analytical Chemistry·Douglas B WeibelGeorge M Whitesides
Aug 2, 2005·Clinical Chemistry·Michael J PugiaLloyd S Schulman
Jan 1, 2004·Proceedings of the IEEE·Peter R C Gascoyne, Jody V Vykoukal

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Citations

Jul 31, 2010·Nano Letters·Tanvir AhmedRoman Stocker
Nov 22, 2007·Lab on a Chip·Daniel IrimiaMehmet Toner
Oct 29, 2008·Chemical Communications : Chem Comm·Jianhua RongQian Wang
Aug 3, 2010·Integrative Biology : Quantitative Biosciences From Nano to Macro·Vijayakrishnan AmbravaneswaranDaniel Irimia
Jul 31, 2009·Journal of Biomechanical Engineering·Hsiu-Hung ChenDayong Gao
Aug 11, 2011·PloS One·Katarina BlagovicJoel Voldman
Apr 28, 2011·Biomicrofluidics·Takahiro ArakawaShuichi Shoji
May 24, 2008·Lab on a Chip·William H GroverScott R Manalis
Sep 25, 2008·Lab on a Chip·Alison M Skelley, Joel Voldman
Nov 22, 2008·Lab on a Chip·He ZhuAlexander Revzin
Aug 2, 2011·Lab on a Chip·Albert J MachDino Di Carlo
May 15, 2013·Lab on a Chip·Ali OskooeiAxel Günther
Oct 23, 2010·Integrative Biology : Quantitative Biosciences From Nano to Macro·Tanvir AhmedRoman Stocker
Nov 28, 2015·Sensors·Jafar Alvankarian, Burhanuddin Yeop Majlis
Feb 12, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Wei-Hua HuangJie-Ke Cheng
Apr 7, 2016·Biomicrofluidics·Michael KlopferMark Bachman
Aug 25, 2009·Analytica Chimica Acta·J NilssonT Laurell
Dec 21, 2006·Lab on a Chip·Kevin R KingMartin L Yarmush
Dec 21, 2007·Lab on a Chip·Halong N VuMartin L Yarmush
Dec 14, 2011·Lab on a Chip·Conrad LochovskyAxel Günther
Aug 2, 2007·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·Richard J GilbertTodd Thorsen
Apr 3, 2020·Soft Matter·Angelo AngeliniFrancesca Frascella
Sep 16, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Haiwa WuJingyao Sun
Dec 4, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kie KasugaCharles N Serhan
Aug 30, 2011·Journal of Micromechanics and Microengineering : Structures, Devices, and Systems·Harrison Prentice-MottDaniel Irimia
Jan 8, 2020·Micromachines·Jun Ho Jang, Ok Chan Jeong
Aug 23, 2019·Lab on a Chip·Juhwan Park, Je-Kyun Park
May 24, 2008·Analytical Chemistry·Jonathan WestAndreas Manz

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