Cell hybridization, hybridomas, and human hybridomas

Methods in Cell Biology
S ShirahataK Teruya

Abstract

Cell hybridization is one of the most basic cytotechnologies. The hemagglutinating virus of Japan was first used to cause cell fusion; however, polyethylene glycol is widely used now because of simplicity of procedure. This chapter first explains the principles of cell hybridization methods and then describes the practical protocols for preparing mouse hybridomas using polyethylene glycol. So far, lack of an excellent human fusion partner cell line that has high fusion efficiencies and does not produce immunoglobulin has hindered the spread of human-human hybridoma preparation methods. In the authors' laboratory NAT-30 and HO-323, human parent cell lines with high fusion efficiencies, have been established to prepare many hybridoma cell lines producing cancer-specific human monoclonal antibodies. Because NAT-30 and HO-323 cell lines are IgM producers, it is difficult to obtain IgG-producing hybridomas because the types of immunoglobulin produced by hybridomas are strongly affected by the characteristics of parent cells. Thus a nonimmunoglobulin-producing human parent cell line, A4H12, derived from human T lymphoma was established that can efficiently obtain IgG-producing human hybridomas. Another problem with preparing human hy...Continue Reading

Citations

Feb 15, 2013·PLoS Pathogens·Mayo YasugiKazuyoshi Ikuta
Jan 31, 2007·Medical & Biological Engineering & Computing·Eduardo Marbán, Hee Cheol Cho
Mar 6, 2010·Circulation Research·Hee Cheol Cho, Eduardo Marbán
Mar 3, 2009·Hybridoma·Reza GolestaniSeyed Mohammad Moazzeni
Feb 19, 2008·Current Opinion in Cardiology·Eduardo Marbán, Hee Cheol Cho

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