Cell-Instructive Microgels with Tailor-Made Physicochemical Properties

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Simone AllazettaM P Lutolf

Abstract

A microfluidic in vitro cell encapsulation platform to systematically test the effects of microenvironmental parameters on cell fate in 3D is developed. Multiple cell types including fibroblasts, embryonic stem cells, and cancer cells are incorporated in enzymatically cross-linked poly(ethylene glycol)-based microgels having defined and tunable mechanical and biochemical properties. Furthermore, different approaches to prevent cell "escape" from the microcapsules are explored and shown to substantially enhance the potential of this technology. Finally, coencapsulation of microgels within nondegradable gels allows cell viability, proliferation, and morphology to be studied in different microenvironmental conditions up to two weeks in culture.

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Citations

May 24, 2016·ACS Applied Materials & Interfaces·Fanfan FuYuanjin Zhao
Mar 24, 2016·Advanced Materials·Erik VrijNicolas Rivron
Dec 16, 2016·Advanced Healthcare Materials·Tom KampermanJeroen Leijten
Jul 13, 2018·Chemical Society Reviews·Wen LiDavid A Weitz
May 24, 2018·Chemistry : a European Journal·Jungkyu K LeeEunAh Lee
May 11, 2016·Annals of Biomedical Engineering·Allen L Liu, Andrés J García
Jul 5, 2019·Biomaterials Science·Laura KolbMatthias P Lutolf
Feb 29, 2020·Biomaterials Science·Barbara Kupikowska-Stobba, Dorota Lewińska
Jun 27, 2020·Soft Matter·Cameron D MorleyThomas E Angelini
Feb 6, 2017·Lab on a Chip·Philipp S LienemannDavid J Mooney
Nov 1, 2016·Lab on a Chip·Weiqian JiangKam W Leong
May 10, 2019·APL Bioengineering·Roger D KammMuhammad H Zaman
Jun 13, 2017·Macromolecular Rapid Communications·S AllazettaM P Lutolf
Apr 7, 2021·Scientific Reports·Anna FornellMaria Tenje
May 6, 2021·Advanced Healthcare Materials·Susan BabuLaura De Laporte
Jun 19, 2021·ACS Applied Materials & Interfaces·Gustavo Ramirez-CalderonCharlotte A E Hauser
Jan 1, 2020·Nature Reviews. Materials·Andrew C DalyJason A Burdick

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