Cell interaction microarray for blood phenotyping

Analytical Chemistry
Colin J CampbellP Ghazal

Abstract

Microarrays promise great advances in areas of diagnostic testing where there is a need to perform multiple assays in parallel. In the short term, protein microarrays have a greater potential to impact diagnostics than DNA arrays due to their potential for direct sample measurements. Here, we report an antibody microarray technique for selectively recognizing glycan and peptide motifs on the surface of red blood cells. We present results demonstrating the optimization and efficacy of the microarray approach as a highly sensitive and specific microscale multiplex assay for blood typing. We also show that our microarray can be used to screen red blood cell surface antigens using whole blood in a label-free detection mode. Finally, our results indicate this method has potential for broader applications in biochip medicine.

References

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Citations

Sep 2, 2011·Analytical and Bioanalytical Chemistry·Stuart W J EmberColin J Campbell
Jun 26, 2010·Biomedical Microdevices·Chin-Hsiung HsiehYi-You Huang
Mar 25, 2008·Journal of Immunoassay & Immunochemistry·Nichola O'LooneyColin J Campbell
Mar 12, 2009·Science in China. Series C, Life Sciences·Fan YangMian Long
May 22, 2009·Journal of Proteomics·Carl A K Borrebaeck, Christer Wingren
Aug 8, 2007·Current Opinion in Biotechnology·Colin J Campbell, K Dane Wittrup
Apr 27, 2007·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Juraj PetrikJerard Seghatchian
Nov 20, 2008·British Journal of Haematology·Neil D Avent
Aug 2, 2006·Transfusion Medicine·J Petrik
Jun 16, 2010·Transfusion·Chantal Fournier-WirthJoliette Coste
Mar 18, 2011·Faraday Discussions·Holger SchulzeColin J Campbell
Sep 26, 2007·Expert Review of Molecular Diagnostics·Carl A K Borrebaeck, Christer Wingren
Jul 9, 2020·Analytical Chemistry·Prerit MathurGovind V Kaigala

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