Cell jamming, stratification and p63 expression in cultivated human corneal epithelial cell sheets.

Scientific Reports
Koichi BabaKohji Nishida

Abstract

Corneal limbal epithelial stem cell transplantation using cultivated human corneal epithelial cell sheets has been used successfully to treat limbal stem cell deficiencies. Here we report an investigation into the quality of cultivated human corneal epithelial cell sheets using time-lapse imaging of the cell culture process every 20 minutes over 14 days to ascertain the level of cell jamming, a phenomenon in which cells become smaller, more rounded and less actively expansive. In parallel, we also assessed the expression of p63, an important corneal epithelial stem cell marker. The occurrence of cell jamming was variable and transient, but was invariably associated with a thickening and stratification of the cell sheet. p63 was present in all expanding cell sheets in the first 9 days of culture, but it's presence did not always correlate with stratification of the cell sheet. Nor did p63 expression necessarily persist in stratified cell sheets. An assessment of cell jamming, therefore, can shed significant light on the quality and regenerative potential of cultivated human corneal epithelial cell sheets.

References

Oct 1, 1995·Ophthalmology·V Puangsricharern, S C Tseng
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·G PellegriniM De Luca
Mar 19, 2002·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·J T DanielsP T Khaw
Jan 20, 2004·Genes & Development·Maranke I KosterDennis R Roop
Sep 28, 2005·Investigative Ophthalmology & Visual Science·Parthasarathy ArpithaVeerappan Muthukkaruppan
Nov 7, 2009·Investigative Ophthalmology & Visual Science·José Alvaro Pereira GomesIrina Kerkis
Jun 25, 2010·The New England Journal of Medicine·Paolo RamaGraziella Pellegrini
Nov 16, 2010·Trends in Molecular Medicine·Graziella PellegriniMichele De Luca
Feb 16, 2011·Proceedings of the National Academy of Sciences of the United States of America·Thomas E AngeliniDavid A Weitz
Sep 6, 2011·The British Journal of Ophthalmology·Virender S SangwanDorairajan Balasubramanian
Jun 25, 2013·Differentiation; Research in Biological Diversity·Monirosadat SadatiJeffrey J Fredberg
Apr 22, 2015·The Journal of Cell Biology·Daisuke NanbaYann Barrandon
Jul 19, 2015·Progress in Retinal and Eye Research·Takahiro NakamuraShigeru Kinoshita
Aug 4, 2015·Nature Materials·Jin-Ah ParkJeffrey J Fredberg
May 28, 2016·Science·Panteleimon RompolasValentina Greco
Nov 9, 2016·Nature Biotechnology·Stavroula SkylakiTimm Schroeder
Apr 1, 2016·Physical Review. X·Dapeng BiM Lisa Manning
May 2, 2018·Soft Matter·Oleksandr ChepizhkoCaterina A M La Porta
May 3, 2019·Contact Lens & Anterior Eye : the Journal of the British Contact Lens Association·Kiranjit K BainsAndrew J Quantock
Nov 9, 2019·Biochemical and Biophysical Research Communications·Jae Hun KimJeffrey J Fredberg

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Methods Mentioned

BETA
fluorescence
x-ray irradiation
FCS

Software Mentioned

Image
Synergy
KaleidaGraph
Image J
Pro Plus

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