Cell-laden alginate dialdehyde-gelatin hydrogels formed in 3D printed sacrificial gel.

Journal of Materials Science. Materials in Medicine
Dalia DranseikieneAldo R Boccaccini

Abstract

Alginate dialdehyde-gelatin (ADA-GEL) hydrogels have been reported to be suitable matrices for cell encapsulation. In general, application of ADA-GEL as bioink has been limited to planar structures due to its low viscosity. In this work, ring shaped constructs of ADA-GEL hydrogel were fabricated by casting the hydrogel into sacrificial molds which were 3D printed from 9% methylcellulose and 5% gelatin. Dissolution of the supporting structure was observed during the 1st week of sample incubation. In addition, the effect of different crosslinkers (Ba2+ and Ca2+) on the physicochemical properties of ADA-GEL and on the behavior of encapsulated MG-63 cells was investigated. It was found that Ba2+ crosslinked network had more than twice higher storage modulus, and mass decrease to 70% during incubation compared to 42% in case of hydrogels crosslinked with Ca2+. In addition, faster increase in cell viability during incubation and earlier cell network formation were observed after Ba2+ crosslinking. No negative effects on cell activity due to the use of sacrificial materials were observed. The approach presented here could be further developed for cell-laden ADA-GEL bioink printing into complex 3D structures.

References

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Aug 5, 2017·Materials·Tobias ZehnderAldo R Boccaccini
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Dec 7, 2018·Journal of Materials Science. Materials in Medicine·Supachai ReakasameAldo R Boccaccini
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Jun 10, 2019·ACS Biomaterials Science & Engineering·Nikoo SoltanXiongbiao Chen

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Citations

Dec 6, 2020·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Fei-Fan CaiAldo R Boccaccini
Feb 14, 2021·Materials·Magdalena B ŁabowskaIzabela Michalak
Nov 13, 2020·Frontiers in Bioengineering and Biotechnology·Benedetta E FornasariStefania Raimondo
Mar 4, 2021·International Journal of Molecular Sciences·Hatice GençIwona Cicha
May 3, 2021·International Journal of Biological Macromolecules·Balaji MahendiranGopal Shankar Krishnakumar

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Methods Mentioned

BETA
infrared spectroscopy
fluorescence microscopy
Light
Fluorescence

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