Cell layer-specific distribution of transiently expressed barley ESCRT-III component HvVPS60 in developing barley endosperm

Protoplasma
Julia HilscherVerena Ibl

Abstract

The significance of the endosomal sorting complexes required for transport (ESCRT)-III in cereal endosperm has been shown by the identification of the recessive mutant supernumerary aleurone layer1 (SAL1) in maize. ESCRT-III is indispensable in the final membrane fission step during biogenesis of multivesicular bodies (MVBs), responsible for protein sorting to vacuoles and to the cell surface. Here, we annotated barley ESCRT-III members in the (model) crop Hordeum vulgare and show that all identified members are expressed in developing barley endosperm. We used fluorescently tagged core ESCRT-III members HvSNF7a/CHMP4 and HvVPS24/CHMP3 and the associated ESCRT-III component HvVPS60a/CHMP5 for transient localization studies in barley endosperm. In vivo confocal microscopic analyses show that the localization of recombinantly expressed HvSNF7a, HvVPS24 and HvVPS60a differs within barley endosperm. Whereas HvSNF7a induces large agglomerations, HvVPS24 shows mainly cytosolic localization in aleurone and subaleurone. In contrast, HvVPS60a localizes strongly at the plasma membrane in aleurone. In subaleurone, HvVPS60a was found to a lesser extent at the plasma membrane and at vacuolar membranes. These results indicate that the steady...Continue Reading

References

Jul 1, 1995·The Plant Cell·P R ShewryA S Tatham
Jan 1, 1993·Methods in Enzymology·J C SanfordJ A Russell
Dec 19, 2001·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Wiebke MöbiusJan W Slot
Dec 4, 2002·Nature Reviews. Molecular Cell Biology·David J KatzmannScott D Emr
May 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·Bo ShenOdd-Arne Olsen
Sep 25, 2003·Cell·Bettina StrackHeinrich G Göttlinger
Oct 30, 2003·The Biochemical Journal·Jeremy W PeckPeter D Burbelo
Mar 11, 2004·The Plant Cell·Odd-Arne Olsen
Oct 27, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jörg WiedenmannG Ulrich Nienhaus
Apr 16, 2005·Current Opinion in Biotechnology·Eva StogerPaul Christou
Feb 21, 2006·Trends in Plant Science·Verena Winter, Marie-Theres Hauser
May 13, 2006·Annual Review of Biophysics and Biomolecular Structure·James H Hurley, Scott D Emr
Nov 9, 2006·Development·Christoph SpitzerMartin Hülskamp
Dec 6, 2006·Proceedings of the National Academy of Sciences of the United States of America·Alessia ZamborliniHeinrich Göttlinger
Apr 24, 2007·Nature Reviews. Molecular Cell Biology·Roger L Williams, Sylvie Urbé
May 18, 2007·Annual Review of Cell and Developmental Biology·Robert C Piper, David J Katzmann
Jan 23, 2008·The Journal of Cell Biology·Phyllis I HansonJohn E Heuser
Sep 16, 2008·Journal of Experimental Botany·Lene T OlsenHilde-Gunn Opsahl-Sorteberg
Nov 7, 2008·Proceedings of the National Academy of Sciences of the United States of America·Ann-Christin LindåsRolf Bernander
Jan 16, 2009·Biochemical Society Transactions·Jeremy G Carlton, Juan Martin-Serrano
Feb 3, 2009·Methods in Molecular Biology·Thomas RademacherEva Stoger
Jun 19, 2009·Journal of Cell Science·Tor Erik Rusten, Harald Stenmark
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Yayoi OndaYasushi Kawagoe
Aug 26, 2009·Communicative & Integrative Biology·Thijs Jg Ettema, Rolf Bernander
Sep 29, 2009·Trends in Microbiology·Rachel Y Samson, Stephen D Bell
Mar 13, 2010·Biology of the Cell·Ingrid RoxrudLene Malerød
May 27, 2010·Plant Biotechnology Journal·Joseph BootheMaurice M Moloney
Jul 1, 2010·Nature Reviews. Molecular Cell Biology·James H Hurley, Phyllis I Hanson
Oct 30, 2010·Trends in Biochemical Sciences·Suman PeelWinfried Weissenhorn
Nov 26, 2010·The Plant Journal : for Cell and Molecular Biology·Yihua WangJianmin Wan

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Datasets Mentioned

BETA
AK376941

Methods Mentioned

BETA
confocal microscopy
transgenic
PCR
confocal
electron microscopy

Software Mentioned

Leica confocal
TBLASTN
POPcorn
ESCRT
MEGA6
IPK Barley BLAST
Adobe Photoshop
Ensembl Plants platform BLAST Server
ImageJ
MUSCLE

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