Cell-line-dependent sorting of recombinant phytase in cell cultures of Medicago truncatula

Functional Plant Biology : FPB
Pablo Gonzalez-MelendiRita Abranches

Abstract

Plant cell cultures as platforms for recombinant protein production are favoured over other systems because they combine the benefits of plants (low cost of production, low biosecurity risk, conserved post-translational modifications) with those of controlled cell cultures. However, many factors that affect the correct synthesis and accumulation of the recombinant product still need to be determined; in particular, the trafficking route of the recombinant proteins is poorly understood. Suspension cell cultures of Medicago truncatula Gaertn. have been shown to offer a viable and highly efficient system for the production of a model glycoprotein - phytase from the fungus Aspergillus niger Tiegh. The present study investigated subcellular protein sorting by immunogold detection of recombinant phytase with an electron microscope in four independent Medicago cell cultures expressing phytase. Two lines contained a C-terminal KDEL targeting signal for retention in the endoplasmic reticulum (ER), and the other two did not and were expected to travel through the secretory route; a high and low expressor were examined for each variant of the protein. A differential subcellular location of phytase was found in the four transgenic lines st...Continue Reading

References

Feb 28, 2002·The Plant Journal : for Cell and Molecular Biology·Pablo González-Melendi, Peter Shaw
Oct 4, 2003·Nature Reviews. Genetics·Julian K-C MaPaul Christou
Jul 17, 2004·The Plant Journal : for Cell and Molecular Biology·Kentaro TamuraIkuko Hara-Nishimura
Sep 24, 2004·Journal of Biotechnology·Diethard MattanovichMichael Sauer
Nov 6, 2004·Nature Biotechnology·Stephan HellwigRainer Fischer
Apr 25, 2006·Plant Physiology·Georgia DrakakakiEva Stoger
Jun 20, 2006·Trends in Plant Science·Sally L HantonFederica Brandizzi
Jul 18, 2006·Trends in Biotechnology·Pauline M Doran
Oct 25, 2006·The Plant Journal : for Cell and Molecular Biology·Eva M KleinAlessandro Vitale
Mar 20, 2008·Journal of Experimental Botany·Pablo González-MelendiM Mercedes Lucas
Jul 17, 2008·Traffic·Ombretta Foresti, Jürgen Denecke
Sep 10, 2008·Journal of Proteome Research·Junling LuoDaichang Yang

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