Cell line specific modulation of connexin43 expression after exposure to ionizing radiation

Cell Communication & Adhesion
Ferya Banaz-YaşarAlexandra Gellhaus

Abstract

Gap junctional intercellular communication plays a significant role in mediating radiation-induced bystander effects. However, the level of Cx43 itself is influenced by ionizing radiation, which could modify the bystander effect. Here we have investigated several cell lines for the modulation of Cx43 expression 24 h after irradiation with 5 Gy X-rays. The mouse endothelial cell line bEnd3 revealed a significantly elevated level of Cx43 already 15 min after exposure to X-rays, whereas human hybrid endothelial cells (EA.hy926) exhibited a transient downregulation of Cx43 mRNA. No obvious changes in the communication properties of the different cell lines could be observed after irradiation. The communication-deficient malignant human trophoblast cell line Jeg3 stably transfected with Cx43 did not reveal any induction of endogenous nor alteration in the exogenous Cx43 transcript level upon exposure to 5 Gy. Taken together, our data show a cell line specific modulation of Cx43 expression after exposure to X-rays.

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Citations

Apr 3, 2008·Journal of Biomedicine & Biotechnology·M Ahmad Chaudhry
Aug 6, 2010·Brain Research Reviews·Filipa Lourenço CardosoMaria Alexandra Brito
May 23, 2007·Journal of Cellular Biochemistry·Ferya Banaz-YaşarAlexandra Gellhaus
Jan 15, 2014·Placenta·D J KanterY Sadovsky
Jan 4, 2021·Cellular and Molecular Life Sciences : CMLS·Raghda RamadanLuc Leybaert

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