Jun 6, 2012

Cell line specific modulation of extracellular aβ42 by Hsp40

PloS One
Anna CarniniJanice E A Braun

Abstract

Heat shock proteins (Hsps) are a set of molecular chaperones involved in cellular repair. They provide protective mechanisms that allow cells to survive potentially lethal insults, In response to a conditioning stress their expression is increased. Here we examined the connection between Hsps and Aβ(42), the amyloid peptide involved in the pathological sequence of Alzheimer's disease (AD). Extracellular Aβ(42) associates with neuronal cells and is a major constituent of senile plaques, one of the hallmarks of AD. Although Hsps are generally thought to prevent accumulation of misfolded proteins, there is a lack of mechanistic evidence that heat shock chaperones directly modulate Aβ(42) toxicity. In this study we show that neither extracellular Aβ(42) nor Aβ(42/)PrP(C) trigger the heat shock response in neurons. To address the influence of the neuroprotective heat shock response on cellular Aβ(42), Western analysis of Aβ(42) was performed following external Aβ(42) application. Five hours after a conditioning heat shock, Aβ(42) association with CAD cells was increased compared to control neurons. However, at forty-eight hours following heat shock Aβ(42) levels were reduced compared to that found for control cells. Moreover, transi...Continue Reading

  • References64
  • Citations2

Citations

Mentioned in this Paper

Senile Paranoid Dementia
Pathologic Cytolysis
HSPB1 gene
Monoclonal Antibodies
Familial Alzheimer Disease (FAD)
Heat shock proteins
Biochemical Pathway
Neurofilament
Transfection
Tissue Membrane

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