PMID: 8605253Feb 7, 1996Paper

Cell line-specific transcriptional activation of the promoter of the human guanylyl cyclase C/heat-stable enterotoxin/receptor gene

Biochimica Et Biophysica Acta
E A MannR A Glenella

Abstract

The guanylyl cyclase C protein, expressed primarily in the intestine, is the receptor for the heat-stable enterotoxin of Escherichia coli. We have isolated and sequenced the promoter region and the first exon of human guanylyl cyclase C and determined the major site of transcription initiation. Transfection of a -1973/+124 promoter/luciferase gene fusion construct in the Caco-2 intestinal cell line resulted in a high level of expression; results with deletion constructs indicate the presence of multiple positive-acting sequence elements. These promoter elements were not active upon transfection into NIH/3T3 and LLC-PK1 cell lines which do not express GC-C.

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Citations

Mar 30, 2001·European Journal of Biochemistry·N RoyS S Visweswariah
Jun 3, 2005·Zoological Science·Mina Nakauchi, Norio Suzuki
Aug 14, 2001·Developmental Dynamics : an Official Publication of the American Association of Anatomists·L A Ehrman, K E Yutzey
Jul 1, 1997·Journal of Receptor and Signal Transduction Research·J P BalintM B Cohen
Oct 7, 2016·Proceedings. Biological Sciences·Brad J GemmellTracy A Villareal
Jul 27, 2007·Orthodontics & Craniofacial Research·I C GayM MacDougall
Dec 5, 2009·Molecular and Cellular Biochemistry·Nirmalya BasuSandhya S Visweswariah
Mar 10, 1999·The American Journal of Physiology·E S SwensonR A Giannella
May 4, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·W ZhangS A Waldman
Nov 14, 1997·The American Journal of Physiology·J A HochmanM B Cohen

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