Cell lineage analyses of epithelia and blood vessels in chimeric mouse embryos by use of an embryonic stem cell line expressing the beta-galactosidase gene

Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists
Y KadokawaN Nakatsuji

Abstract

We have established an embryonic stem (ES) cell line, MS1-EL4, which has the potential to make various tissues in chimeric embryos and, at the same time, expresses the beta-galactosidase gene which was introduced as a good cell marker. To examine cell behavior and lineage during embryogenesis, we injected MS1-EL4 cells into host blastocysts and recovered chimeric embryos at various developmental stages. We examined the distribution of the MS1-EL4 cell derivatives by staining whole embryos with X-gal and by making serial paraffin sections. So far we have obtained the following results: (1) the MS1-EL4 cell line is useful for studying cell lineages because of its ubiquitous expression at least until the mid-gestation stage; (2) cells of the primitive ectoderm and its derivative epithelial tissues continue to intermingle with each other until the late primitive streak stage. Then, at early somite stages, cells of various epithelia stop intermingling and give rise to small coherent clones; (3) blood vessels of the yolk sac are formed by local aggregation of the ancestor cells and those of the embryo proper by proliferation and sprouting from fewer angiogenic cells.

References

Mar 1, 1990·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·H SuemoriN Nakatsuji
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J PriceC Cepko
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Citations

May 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·D J DumontM L Breitman
Jan 13, 2006·Cell and Tissue Research·Paul E MozdziakJames N Petitte
Oct 1, 1996·Cytokine & Growth Factor Reviews·M Klagsbrun, P A D'Amore
Oct 20, 2005·Journal of Bioscience and Bioengineering·Ken-Ichiro OnoShinji Iijima
Mar 6, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·P E MozdziakJ N Petitte
Sep 21, 2002·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Yuzo Kadokawa, Tohru Marunouchi
Sep 1, 1997·Developmental Dynamics : an Official Publication of the American Association of Anatomists·O CleaverP A Krieg

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