Cell lineage-specific genome-wide DNA methylation analysis of patients with paediatric-onset systemic lupus erythematosus

Epigenetics : Official Journal of the DNA Methylation Society
Kit San YeungBrian Hon Yin Chung

Abstract

Patients with paediatric-onset systemic lupus erythematosus (SLE) often present with more severe clinical courses than adult-onset patients. Although genome-wide DNA methylation (DNAm) profiling has been performed in adult-onset SLE patients, parallel data on paediatric-onset SLE are not available. Therefore, we undertook a genome-wide DNAm study in paediatric-onset SLE patients across multiple blood cell lineages. The DNAm profiles of four purified immune cell lineages (CD4 + T cells, CD8 + T cells, B cells and neutrophils) and whole blood were compared in 16 Chinese patients with paediatric-onset SLE and 13 healthy controls using the Illumina HumanMethylationEPIC BeadChip. Comparison of DNAm in whole blood and within each independent cell lineage identified a consistent pattern of loss of DNAm at 21 CpG sites overlapping 15 genes, which represented a robust, disease-specific DNAm signature for paediatric-onset SLE in our cohort. In addition, cell lineage-specific changes, involving both loss and gain of DNAm, were observed in both novel genes and genes with well-described roles in SLE pathogenesis. This study also highlights the importance of studying DNAm changes in different immune cell lineages rather than only whole blood...Continue Reading

Citations

Jun 26, 2020·Frontiers in Cell and Developmental Biology·Gangqiang GuoXiangyang Xue
Jul 25, 2019·Frontiers in Immunology·Anna Elisa Andrea Surace, Christian M Hedrich
Sep 29, 2020·Frontiers in Immunology·Maria J GutierrezXiaobin Wang

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Methods Mentioned

BETA
PCA
blood draw
flow cytometry

Software Mentioned

R
GeneMANIA
minfi
SPSS Statistics
Qlucore Omics Explorer

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