Cell Mass Increase Associated with Formation of Glucose-Controlling β-Cell Mass in Device-Encapsulated Implants of hiPS-Derived Pancreatic Endoderm

Stem Cells Translational Medicine
Thomas RobertDaniel G Pipeleers

Abstract

Device-encapsulated human stem cell-derived pancreatic endoderm (PE) can generate functional β-cell implants in the subcutis of mice, which has led to the start of clinical studies in type 1 diabetes. Assessment of the formed functional β-cell mass (FBM) and its correlation with in vivo metabolic markers can guide clinical translation. We recently reported ex vivo characteristics of device-encapsulated human embryonic stem cell-derived (hES)-PE implants in mice that had established a metabolically adequate FBM during 50-week follow-up. Cell suspensions from retrieved implants indicated a correlation with the number of formed β cells and their maturation to a functional state comparable to human pancreatic β cells. Variability in metabolic outcome was attributed to differences in number of PE-generated β cells. This variability hinders studies on processes involved in FBM-formation. This study reports modifications that reduce variability. It is undertaken with device-encapsulated human induced pluripotent stem cell-derived-PE subcutaneously implanted in mice. Cell mass of each cell type was determined on intact tissue inside the device to obtain more precise data than following isolation and dispersion. Implants in a preformed ...Continue Reading

References

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Citations

Apr 2, 2020·Stem Cells·Stuart P Atkinson
Aug 28, 2020·Stem Cell Reviews and Reports·Mitchell H MaloyNatesh Parashurama
Nov 19, 2020·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Freya Van HulleDaniel Pipeleers
Aug 23, 2020·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·Sara J PhotiadisDeepika Sarode

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ViaCyte
Pathomation
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SmartCapture

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