Cell-matrix interactions improve beta-cell survival and insulin secretion in three-dimensional culture.

Tissue Engineering. Part a
Laney M WeberKristi S Anseth

Abstract

Controlled matrix interactions were presented to pancreatic beta-cells in three-dimensional culture within poly(ethylene glycol) hydrogels. Dispersed MIN6 beta-cells were encapsulated in gel environments containing the following entrapped extracellular matrix (ECM) proteins: collagen type I, collagen type IV, fibrinogen, fibronectin, laminin, and vitronectin. In ECM-containing gels, beta-cell survival was significantly better than in gels without ECM over 10 days. Correspondingly, apoptosis in encapsulated beta-cells was less in the presence of each matrix protein, suggesting the ability of individual matrix interactions to prevent matrix signaling-related apoptosis (anoikis). MIN6 beta-cells cultured in gels containing collagen type IV or laminin secreted more insulin in response to glucose stimulation than beta-cells in all other experimental conditions. Variations in collagen type IV or laminin concentration between 10 microg/mL and 250 microg/mL did not affect insulin secretion. Finally, beta-cell function in hydrogels presenting both collagen type IV and laminin revealed synergistic interactions. With a total protein concentration of 100 microg/mL, three gel compositions of varying ratios of collagen type IV to laminin (25...Continue Reading

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Citations

Apr 16, 2013·Biomaterials·Sayed-Hadi Mirmalek-SaniShay Soker
Apr 6, 2011·Proceedings of the National Academy of Sciences of the United States of America·Chien-Chi Lin, Kristi S Anseth
Sep 26, 2012·Tissue Engineering. Part a·Karthik RamachandranLisa Stehno-Bittel
Apr 12, 2011·Tissue Engineering. Part B, Reviews·Jennifer Y C ChengLaura Poole-Warren
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Methods Mentioned

BETA
nuclear magnetic resonance
Assay
confocal microscopy
enzyme-linked immunosorbent assay
ELISA

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